| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We successfully analyzed CSF1R [pro-tumoral M2-like tumor associated macrophages(TAMs)], immune regulatory M2c-like TAMs (CD163, PTX3, IL10, TGFB) and other markers of immune microenvironment in Diffuser Large B-cell Lymphoma (DLBCL, 240 cases, 29 sinonasal) and Follicular Lymphoma (FL, 100 Japan, 88 Europe). We performed (1) Immunohistochemistry and digital image quantification (2) Immunofluorescence, optical, confocal and atomic force microscopy (3) Genome-wide copy number and loss-of-heterozygosity (LOH) profiling (4) In vitro analysis of M2 macrophages + FL with gene expression profiling, including GSEA and network analysis (5) Correlation with histological and clinical features. We concluded that high CSF1R and M2c-like markers correlated with FL progression and DLBCL transformation; TAMs created 3D networks, TAMs induced changes in gene expression of immune response type II, high TAMs associated with poor prognosis in DLBCL and TAMs correlated with high copy No changes and RGS1.
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