Colony stimulating factor 1 receptor (CSF1R) and immune regulatory M2c-like tumor associated macrophages (TAMs) in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma transformation (tFL)
| Project/Area Number |
15K19061
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | CSF1R / びまん性大細胞型B細胞リンパ腫 / 濾胞性リンパ腫 / 腫瘍関連マクロファージ / 血液病理学 / 免疫組織化学 / 全ゲノムのコピー数解析 / CD163 / 全ゲノム解析 / 病理学 / 血液 / びまん性大細胞型Ｂ細胞性リンパ腫 / ＣＳＦ１Ｒ / マクロファージ |
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| Outline of Final Research Achievements |
We successfully analyzed CSF1R [pro-tumoral M2-like tumor associated macrophages(TAMs)], immune regulatory M2c-like TAMs (CD163, PTX3, IL10, TGFB) and other markers of immune microenvironment in Diffuser Large B-cell Lymphoma (DLBCL, 240 cases, 29 sinonasal) and Follicular Lymphoma (FL, 100 Japan, 88 Europe). We performed (1) Immunohistochemistry and digital image quantification (2) Immunofluorescence, optical, confocal and atomic force microscopy (3) Genome-wide copy number and loss-of-heterozygosity (LOH) profiling (4) In vitro analysis of M2 macrophages + FL with gene expression profiling, including GSEA and network analysis (5) Correlation with histological and clinical features. We concluded that high CSF1R and M2c-like markers correlated with FL progression and DLBCL transformation; TAMs created 3D networks, TAMs induced changes in gene expression of immune response type II, high TAMs associated with poor prognosis in DLBCL and TAMs correlated with high copy No changes and RGS1.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Clinicopathological characteristics and genomic profile of primary sinonasal tract diffuse large B cell lymphoma (DLBCL) reveals gain at 1q31 and RGS1 encoding protein; high RGS1 immunohistochemical expression associates with poor overall survival in DLBCL not otherwise specified (NOS).2017
Author(s)
Carreras J, Kikuti YY, Bea S, Miyaoka M, Hiraiwa S, Ikoma H, Nagao R, Tomita S, Martin-Garcia D, Salaverria I, Sato A, Ichiki A, Roncador G, Garcia JF, Ando K, Campo E, Nakamura N.
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Journal Title
Histopathology
Volume: 70
Issue: 4
Pages: 595-621
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] RGS1陽性DLBCLの臨床病理学的特徴とゲノムプロファイル解析2017
Author(s)
Carreras Joaquim, 菊池イアーラ 幸江, 宮岡 雅, 平岩 信一郎, 冨田 さくら, Perez Patricia, Bea Silvia, 安藤 潔, Campo Elias, 中村 直哉
Organizer
第57回日本リンパ網内系学会総会
Place of Presentation
京王プラザホテル 〒160-8330 東京都新宿区西新宿2-2-1
Year and Date
2017-06-29
Related Report
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