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Detection of specific antibody-producing cells with the enzyme-labeled antigen method in paraffin sections

Research Project

Project/Area Number 15K19063
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionFujita Health University

Principal Investigator

Mizutani Yasuyoshi  藤田保健衛生大学, 医学部, 助教 (10546229)

Research Collaborator TSUTSUMI Yutaka  
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords酵素抗原法 / 抗体 / 抗原 / 病変局所 / 形質細胞 / パラフィン切片 / B細胞 / ホルマリン固定パラフィン包埋切片 / 病変部
Outline of Final Research Achievements

The purpose of the present study was the application of the enzyme-labeled antigen method in formalin-fixed paraffin-embedded (FFPE) tissue sections. This purpose has not been achieved during this period. However, we elucidated factors inactivating antibodies during paraffin sectioning for the enzyme-labeled antigen method. Fixation methods maintaining antibody activities in paraffin sections were found. We demonstrated properties of paraffin wax used for embedding tissues involve antibody activities in tissue sections. These findings would help us to realize the application of the enzyme-labeled antigen method in FFPE sections.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Presentation (7 results)

  • [Presentation] 酵素抗原法の技術開発:パラフィン包埋切片において固定法が抗体活性におよぼす影響2017

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤 寬
    • Organizer
      第106回日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2017-04-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] Helicobacter pylori感染スナネズミにおける抗Helicobacter pylori抗体産生細胞の局在証明2016

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤寬.
    • Organizer
      第48回藤田学園医学会
    • Place of Presentation
      藤田保健衛生大学(豊明市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酵素抗原法によるピロリ菌感染スナネズミにおける抗ピロリ菌抗体産生細胞の局在証明2016

    • Author(s)
      水谷泰嘉、春宮覚、川久保雅友、中山淳、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤寬
    • Organizer
      第57回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      杏林大学井の頭キャンパス(三鷹市)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酵素抗原法の技術開発:ホルマリン固定パラフィン包埋切片作製工程が抗体活性におよぼす影響2016

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤 寛
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Year and Date
      2016-05-14
    • Related Report
      2015 Research-status Report
  • [Presentation] 酵素抗原法の技術開発 ホルマリン固定パラフィン包埋切片作製過程における組織内の抗体失活要因の検証2015

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤 寛
    • Organizer
      第56回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      関西医科大学・枚方キャンパス(枚方市)
    • Year and Date
      2015-10-04
    • Related Report
      2015 Research-status Report
  • [Presentation] 病変部で産生される抗体の標的抗原検索法と酵素抗原法の技術開発2015

    • Author(s)
      水谷泰嘉
    • Organizer
      第56回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      関西医科大学・枚方キャンパス(枚方市)
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] ホルマリン固定パラフィン包埋切片作製過程における抗体活性失活要因の検証~酵素抗原法の応用をめざして~2015

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、櫻井浩平、稲田健一、堤 寛
    • Organizer
      第47回藤田学園医学会
    • Place of Presentation
      藤田保健衛生大学(豊明市)
    • Year and Date
      2015-10-02
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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