Research Project
Grant-in-Aid for Young Scientists (B)
We performed siRNA-based screening for silencing 18 Rab genes that are related to the vesicle transport from the ER to the plasma membrane. The siRNA screen revealed that KD of Rab8b significantly decreased VLP release efficiency compared with control siRNA-transfected cells. RNAi analysis revealed that Rab8b plays a role in WNV particle release. The amount of WNV particles in the supernatant of Rab8b-deficient cells was significantly decreased compared with that of wild-type cells. We also demonstrated that WNV particles accumulated in the recycling endosomes in WNV-infected cells. In summary, these results suggest that Rab8b is involved in trafficking of WNV particles from recycling endosomes to the plasma membrane.
All 2016 2015
All Journal Article (3 results) (of which Int'l Joint Research: 2 results, Peer Reviewed: 3 results, Open Access: 2 results, Acknowledgement Compliant: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results)
Virus Res.
Volume: 228 Pages: 114-123
10.1016/j.virusres.2016.11.029
J Virol Methods.
Volume: 240 Pages: 14-20
10.1016/j.jviromet.2016.11.006
The Journal of Biological Chemistry
Volume: 291 Issue: 12 Pages: 6559-6568
10.1074/jbc.m115.712760
120005906784
