The role of Opn-expressing CD8 T cells in the intestine

• Project/Area Number: 15K19075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Tohoku University (2016); Kyoto University (2015)
• Principal Investigator: Ito Koyu 東北大学, 加齢医学研究所, 助教 (90609497)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オステオポンチン / 腸内細菌叢 / 腸管上皮内リンパ球

Outline of Final Research Achievements

In this study, using Opn-EGFP knock-in (KI) mice we found that CD8α+ T cells in the intestinal tissues, including Peyer’s patch, lamina propria and epithelium, express Opn under steady state conditions. Among these cell, intraepithelial lymphocyte (IEL) CD8 T cells shows highest expression of Opn, and majority of these cells are TCRγδ cells. Opn knockout (KO) mice had altered fecal microflora concordant with a reduction of TCRγδ+ IELs. Consistent with this result, both treatment with anti-Opn blocking antibody and deficiency of Opn resulted in decreased survival of TCRγδ+ and TCRαβ+ IELs. This data suggests that a possibility that Opn may function as a survival factor for IELs in the intestinal tissue.

Research Products

• [Journal Article] The potential role of Osteopontin in the maintenance of commensal bacteria homeostasis in the intestine. (2017)
  • Author(s): Ito K, Nakajima A1, Fukushima Y1, Suzuki K1, Sakamoto K, Hamazaki Y, Ogasawara K, Minato N, and Hattori M.
  • Journal Title: PLoS One. Volume: 12 Issue: 3 Pages: e0173629-e0173629
  • DOI: 10.1371/journal.pone.0173629
  • NAID: 120006488574
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Osteopontin in spontaneous germinal centers inhibits apoptotic cell engulfment and promotes autoantibody production in lupus mice. (2016)
  • Author(s): Sakamoto、K., Fukushima、Y., Itoh、K., Matsuda, M., Nagata, S., Minato, N., and Hattori, M.
  • Journal Title: J. Immunol. Volume: 197 Issue: 6 Pages: 2177-2186
  • DOI: 10.4049/jimmunol.1600987
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Osteopontin-expressing TCRγδ+ CD8+ intraepithelial lymphocyte maintains homeostasis of commensal bacteria in the intestine (2015)
  • Author(s): 伊藤甲雄、福島祐二、坂本恵子、湊長博、服部雅一
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター (北海道札幌市)
  • Year and Date: 2015-11-18
• [Presentation] 腸管に常在するOpn発現T細胞群の機能解析 (2015)
  • Author(s): 伊藤甲雄、福島祐二、坂本恵子、服部雅一
  • Organizer: 第三回 マトリセルフォーラム
  • Place of Presentation: 三重大学マトリックスバイオロジー研究センター (三重県津市)
  • Year and Date: 2015-09-12