Structural Analysis of Non Mevalonate Pathway Enzyme Groups aiming at Development of Novel Antimalarial Drugs

Research Project

Project/Area Number	15K19086
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Parasitology (including sanitary zoology)
Research Institution	Yokohama College of Pharmacy
Principal Investigator	UEMDA TOMONOBU 横浜薬科大学, 薬学部, 講師 (80514471)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	マラリア / 非メバロン酸経路 / X線結晶構造解析 / 目的タンパク質の発現系の構築
Outline of Final Research Achievements	To lead to the development of novel antimalarial drugs, we aimed to clarify the three-dimensional structure of the non-mevalonate pathway group derived from Plasmodium falciparum, establishing the expression of the target protein, examining the purification conditions, and conducting a crystallization experiment. Although it could not clarify the structure, we succeeded in large-scale purification for several target proteins and screened for crystallization conditions.

Report

(4 results)