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Analysis of the mitosomal fission mechanism in Entamoeba histolytica

Research Project

Project/Area Number 15K19087
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Parasitology (including sanitary zoology)
Research InstitutionTokai University

Principal Investigator

MAKIUCHI Takashi  東海大学, 医学部, 助教 (80587709)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords寄生虫 / 赤痢アメーバ / 原虫 / ミトコンドリア / ミトコンドリア関連オルガネラ / マイトソーム / ダイナミン / オルガネラ / 縮退進化 / 複合体 / ダイナミン関連タンパク質 / 進化
Outline of Final Research Achievements

Entamoeba histolytica is an anaerobic parasitic protist and possesses mitosomes, one of the most highly divergent mitochondrion-related organelles. Although unique metabolism and protein/metabolite transport machinery have been demonstrated in Entamoeba mitosomes, the mechanism of mitosomal fusion and fission remains to be elucidated. In this study, we demonstrate that two dynamin-related proteins (DRPs) are cooperatively involved in the fission of Entamoeba mitosomes. Expression of a dominant negative form of EhDrpA and EhDrpB, and alternatively, repression of gene expression of EhDrpA and EhDrpB genes, caused elongation of mitosomes, reflecting inhibition of mitosomal fission. Moreover, EhDrpA and EhDrpB formed an unprecedented hetero-oligomeric complex with an approximate 1:2 to 1:3 ratio, suggesting that the observed elongation of mitosomes is likely caused by the disruption and instability of the complex caused by an imbalance in the two DRPs.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant.2017

    • Author(s)
      Kazama M, Ogiwara S, Makiuchi T, Yoshida K, Nakada-Tsukui K, Nozaki T, Tachibana H
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 44273-44273

    • DOI

      10.1038/srep44273

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ミトコンドリアの多様な進化 赤痢アメーバマイトソームから見えるミトコンドリアのタンパク質輸送と代謝の進化2016

    • Author(s)
      牧内 貴志、野崎 智義
    • Journal Title

      遺伝 : 生物の科学

      Volume: 70(2) Pages: 93-98

    • Related Report
      2015 Research-status Report
  • [Presentation] 赤痢アメーバの特殊化ミトコンドリアにおける分裂機構の解析2016

    • Author(s)
      牧内 貴志、Herbert J. Santos、野崎 智義、橘 裕司
    • Organizer
      第85回寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県・宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 赤痢アメーバの特殊化ミトコンドリアにおける分裂機構の解析2015

    • Author(s)
      牧内 貴志、Herbert J. Santos、野崎 智義、橘 裕司
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸国際展示場(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 赤痢アメーバのミトコンドリア関連オルガネラ2015

    • Author(s)
      牧内 貴志、見市 文香、Herert J. Santos、今井 賢一郎、戸澤 譲、橘 裕司、野崎 智義
    • Organizer
      第48回日本原生生物学会大会
    • Place of Presentation
      国立感染症研究所(東京都・新宿区)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Analysis of the fission mechanism of mitosomes in Entamoeba histolytica2015

    • Author(s)
      Takashi Makiuchi, Herbert J. Santos, Tomoyoshi Nozaki, & Hiroshi Tachibana
    • Organizer
      XVIII Seminar on Amebiasis 2015
    • Place of Presentation
      Campeche (Mexico)
    • Year and Date
      2015-10-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The highly divergent mitochondrion-related organelle “mitosome” in Entamoeba histolytica2015

    • Author(s)
      Takashi Makiuchi
    • Organizer
      2nd International Symposium Matryoshka-type Evolution of Eukaryotic Cells
    • Place of Presentation
      筑波大学大学会館(茨城県・つくば市)
    • Year and Date
      2015-09-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2018-03-22  

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