Development of mutator Plasmodium falciparum and its application for mutants generation.
| Project/Area Number |
15K19089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Honma Hajime 東京女子医科大学, 医学部, 助教 (10617468)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | マラリア / Plasmodium falciparum / MSH2 / ミスマッチDNA修復 / CRISPR/Cas9 / ミューテーター / ミスマッチ / CRISPR/Cas / MLH1 / Plasmodium |
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| Outline of Final Research Achievements |
MSH2-1 is thought to be one of the major DNA mismatch repair proteins of Plasmodium species. The MSH2-1 mutant of P. falciparum 3D7 was generated by substituting the 513rd proline to threonine with CRISPR/Cas9 based gene editing. The parent strain 3D7 and the MSH2-1 mutant were maintained by the continuous in vitro culture for around 200 days, and then spontaneous mutations were genome-widely evaluated by high-throughput sequencing. Increases of insertion/deletion mutations in microsatellites and structural variants in subtelomeric regions were observed in the MSH2-1 mutant.
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Report
(4 results)
Research Products
(9 results)
