Analysis of the mannnoprotein that is involved in the host intestinal attachment in Candida glabrata

• Project/Area Number: 15K19090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Chiba University
• Principal Investigator: michiyo Sato 千葉大学, 真菌医学研究センター, 特任助教 (70525386)
• Research Collaborator: CHIBANA hiroji 千葉大学, 真菌医学研究センター, 准教授 ; TAKAHASHI azusa 千葉大学, 真菌医学研究センター, 技術職員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Candida glabrata / 病原性因子 / マンナンタンパク質 / カンジダ・グラブラータ / GPIアンカー型タンパク質 / マンナンたんぱく質

Outline of Final Research Achievements

Candida pathogens cause serious opportunistic infections to immunodeficient patients. Candida glabrata is particular problem among Candida pathogens, because its frequency of the isolations from patients tends to increase year by year. In this study, to reveal the infection mechanism of Candida glabrata through the host intestinal mucosa, we performed the screening of the factor that is involved in the adhesion to the host intestinal mucosa. We constructed the screening system to select the gene-disrupted strains by analyzing the samples isolated from the intestinal mucosa of the mice which were ingested the library composed various gene-disrupted strains with a next-generation sequencer. Though we cannot identify the factor involved in the adhesion to the host intestinal mucosa, we identified one of the the enzyme for ergosterol biosynthesis as the virulence factor that can become the useful target for the development of antifungal drugs.

Research Products

• [Journal Article] Comparative genomic and transcriptomic analyses unveil novel features of azole resistance and adaptation to the human host in Candida glabrata (2018)
  • Author(s): Salazar S, Wang C, Munsterkotter M, Okamoto M, Takahashi-Nakaguchi A, Chibana H, Lopes M, Guldener U, Butler G, Mira N.
  • Journal Title: FEMS Yeast Research Volume: 18 Issue: 1
  • DOI: 10.1093/femsyr/fox079
  • Peer Reviewed
• [Journal Article] Cooperation between ER stress and calcineurin signaling contributes to the maintenance of cell wall integrity in Candida glabrata. (2018)
  • Author(s): Tanaka Y, Sasaki M, Ito F, Aoyama T, Sato-Okamoto M, Takahashi-Nakaguchi A, Chibana H, Shibata N
  • Journal Title: Fungal Biology Volume: 122 Issue: 1 Pages: 19-33
  • DOI: 10.1016/j.funbio.2017.09.007
  • Peer Reviewed
• [Journal Article] Convenient method for better preservation of fine structures of cultured macrophages and engulfed yeast cells by freeze-substitution fixation. (2017)
  • Author(s): Yamaguchi M, Takahashi-Nakaguchi A, Aida Y, Sato-Okamoto M, Chibana H.
  • Journal Title: Microscopy (Oxf) Volume: 18 Issue: 3 Pages: 1-3
  • DOI: 10.1093/jmicro/dfx006
  • Peer Reviewed / Open Access
• [Journal Article] The CgHaa1-Regulon Mediates Response and Tolerance to Acetic Acid Stress in the Human Pathogen Candida glabrata. (2016)
  • Author(s): Ruben T Bernardo, Diana V Cunha, Can Wang, Leonel Pereira, Sand 243;nia Silva, Sara B Salazar, Markus S Schr246;der, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Hiroji Chibana, Toshihiro Aoyama, Isabel S225;-Correia, Joana Azeredo, Geraldine Butler, Nuno Pereira Mira
  • Journal Title: Genomes Genetics Volume: 7(1) Issue: 1 Pages: 1-18
  • DOI: 10.1534/g3.116.034660
  • Peer Reviewed