Molecular analysis of the microbial substitution of Staphylococcus aureus and Pseudomonas aeruginosa

• Project/Area Number: 15K19094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: Hiroshima University
• Principal Investigator: Kayama Shizuo 広島大学, 医歯薬保健学研究院(歯), 助教 (50432761)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 日和見感染症 / 緑膿菌 / 黄色ブドウ球菌 / 混合感染 / ランダム変異 / 菌交代現象 / トランスポゾン変異株

Outline of Final Research Achievements

In order to find the resistant facters of Staphylococcus aureus against Pseudomonas aeruginosa, we made transposon mutagenesis library of community-acquired S. aureus, TY825. We found 16 candidate genes that might be related to the resistance facter against P. aeruginosa.

Research Products

• [Journal Article] Inhibitory effects of antibiofilm compound 1 against Staphylococcus aureus biofilms. (2016)
  • Author(s): Shrestha L, Kayama S, Sasaki M, Kato F, Hisatsune J, Tsuruda K, Koizumi K, Tatsukawa N, Yu L, Takeda K, Sugai M.
  • Journal Title: Microbiology and Immunology Volume: 60 Issue: 3 Pages: 148-159
  • DOI: 10.1111/1348-0421.12359
  • NAID: 40020779260
  • Peer Reviewed
• [Journal Article] Persistence and Epidemic Propagation of a Pseudomonas aeruginosa Sequence Type 235 Clone Harboring an IS26 Composite Transposon Carrying the blaIMP-1 Integron in Hiroshima, Japan, 2005 to 2012 (2015)
  • Author(s): Wataru Shimizu, Shizuo Kayama, Shuntaro Kouda, Yoshitoshi Ogura, Kanao Kobayashi, Norifumi Shigemoto, Norimitsu Shimada, Raita Yano, Junzo Hisatsune, Fuminori Kato, Tetsuya Hayashi, Taijiro Sueda, Hiroki Ohge, and Motoyuki Sugai
  • Journal Title: Antimicrobial Agents and Chemotherapy Volume: 59 Issue: 5 Pages: 2678-2687
  • DOI: 10.1128/aac.04207-14
  • Peer Reviewed