| Project/Area Number |
15K19098
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 病原真菌 / アスペルギルス / PAMPs / 感染防御 / 真菌PAMPｓ / α-1,3-グルカン / 真菌細胞壁多糖 / 細胞壁多糖 / グルカン |
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| Outline of Final Research Achievements |
Aspergillus is a medically important fungal genus that can cause a life-threatening infection called aspergillosis in immunocompromised patients. The α-1,3-glucan is the one of main components of Aspergillus cell wall. However, the immunological activity of α-1,3-glucan is not fully characterized. In this study, we prepared soluble and insoluble Aspergillus cell wall α-1,3-glucanand examined the immunological activity.
The Aspergillus cell wall insoluble and soluble α-1,3-glucan (AspAG and ASAG) was prepared from acetone-dried mycerium. AspAG induced inflammatory cytokine production in a dose-dependent manner, but ASAG did not in vitro. AspAG induced inflammatory response such as increased vascular permeability and priming effect to PAMPs in vivo. Anti-ASAG antibody was detected in the serum of each human volunteer. From these findings, Aspergillus cell wall α-1,3-glucan is important molecule in the pathology of Aspergillus related infectious diseases.
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