Fim3-mediated autoagglutination of Bordetella pertussis

• Project/Area Number: 15K19101
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Otsuka Nao 国立感染症研究所, 細菌第二部, 主任研究官 (90596610)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 百日咳菌 / 自己凝集 / 線毛

Outline of Final Research Achievements

Bordetella pertussis is the etiologic agent of whooping cough. It was known that some B. pertussis isolates cause auto-agglutination (Agg) in vitro, however the cause of Agg has not been elucidated. In this study, whole genome sequencing and immunoblot analysis of Agg and non-Agg isolates revealed that highly production of type 3 fimbriae (Fim3) is the main cause of Agg. On the other hand, Agg isolate (Fim3 high production) showed significantly higher ability of lung adhesion than non-Agg strains (fim3-deficient or Fim3 low production strains) in mouse adherence assay, indicating that highly Fim3 production has benefit for in vivo adherence.

Research Products

• [Journal Article] The length of poly(C) stretch in the Bordetella pertussis Pfim3 promoter determines the vag or vrg function of the fim3 gene (2017)
  • Author(s): Otsuka N, Guiso N, Bouchez V
  • Journal Title: Microbiology Volume: 163 Issue: 9 Pages: 1364-1368
  • DOI: 10.1099/mic.0.000514
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Novel IgM-capture enzyme-linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis infection (2016)
  • Author(s): Otsuka N, Gotoh K, Nishimura N, Ozaki T, Nakamura Y, Haga K, Yamazaki M, Gondaira F, Okada K, Miyaji Y, Toyoizumi-Ajisaka H, Shibayama K, Arakawa Y, Kamachi K.
  • Journal Title: Microbiol Immunol. Volume: 60 Issue: 5 Pages: 326-333
  • DOI: 10.1111/1348-0421.12378
  • Peer Reviewed
• [Presentation] Fimbriae-mediated autoagglutination of Bordetella pertussis (2016)
  • Author(s): 大塚菜緒、平松征洋、蒲地一成、柴山恵吾
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2016-03-24
• [Presentation] 国立感染症研究所・細菌第二部における業務と薬学の関わり (2015)
  • Author(s): 大塚菜緒
  • Organizer: 第27回微生物シンポジウム
  • Place of Presentation: 岡山県：就実大学
  • Year and Date: 2015-09-05
  • Invited
• [Book] 感染 炎症 免疫 (2015)
  • Author(s): 大塚菜緒、蒲地一成
  • Total Pages: 11
  • Publisher: 鳥居薬品株式会社