| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Bordetella pertussis is the etiologic agent of whooping cough. It was known that some B. pertussis isolates cause auto-agglutination (Agg) in vitro, however the cause of Agg has not been elucidated. In this study, whole genome sequencing and immunoblot analysis of Agg and non-Agg isolates revealed that highly production of type 3 fimbriae (Fim3) is the main cause of Agg. On the other hand, Agg isolate (Fim3 high production) showed significantly higher ability of lung adhesion than non-Agg strains (fim3-deficient or Fim3 low production strains) in mouse adherence assay, indicating that highly Fim3 production has benefit for in vivo adherence.
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