| Project/Area Number |
15K19104
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Murakami Shin 東京大学, 大学院農学生命科学研究科(農学部), 助教 (10636757)
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| Research Collaborator |
NODA Takeshi 京都大学, ウイルス再生医学研究所, 教授
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | A型インフルエンザウイルス / ゲノムパッケージング / インフルエンザウイルス |
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| Outline of Final Research Achievements |
By serial passaging of PB2 segment deficient virus (ΔPB2 virus) in PB2 stably expressing MDCK cells, we obtained 3 strains ofΔPB2 virus with high growth property. All the 3 strains possessed mutations in their NP proteins. The NP mutations caused sifting the charge to basic side.
Although ΔHA virus lacked its HA segment, ΔHA virus possessed 8 genome segment in its virion. Next generation sequencing and Northern blotting analysis revealed that ΔHA virus incorporated host-derived rRNA in its virion. These findings highlight the importance of the assembly of eight RNPs in progeny virions for genome packaging.
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