Study of the host factor important for secondary envelopment of HSV-1

• Project/Area Number: 15K19108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Tokyo Metropolitan Institute of Medical Science (2016); The University of Tokyo (2015)
• Principal Investigator: KOBAYASHI Kyousuke 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80644989)
• Research Collaborator: IBA Hideo 千葉大学, 真菌医学研究センター, 教授 ; SAGARA Hiroshi 東京大学, 医科学研究所, 助教
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ヘルペスウイルス / miRNA / ゴルジ体 / エンベロープ形成 / ウイルス学 / Rho GTPase / ウイルス

Outline of Final Research Achievements

miRNAs regulate gene expression post transcriptionally. MiR-199a-3p, a kind of miRNA, reportedly impairs replication of various viruses. These evidences suggested that miR-199a-3p targets host factors important for replication of various virus. We here aimed to identify such host factors targeted by miR-199a using Herpes simplex virus-1 (HSV-1) as a model system. We identified human ARHGAP21 as a target of both miR-199a-5p and miR-199a-3p. Our results suggested that this molecule regulate the function of the Golgi apparatus and secondary envelopment of HSV-1 at the trans cisternae of the Golgi apparatus.

Research Products

• [Journal Article] 1.Pull-down method to access the cell surface receptor for Toxoplasma gondii. (2016)
  • Author(s): Gong H, Kobayashi K, Sugi T, Takemae H, Horimoto T, Xuan X, Akashi H, Kato K
  • Journal Title: Parasitol. Int. Volume: 65 Issue: 5 Pages: 514-515
  • DOI: 10.1016/j.parint.2016.08.013
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 2.Evaluating the use of heparin for synchronization of in vitro culture of Plasmodium falciparum. (2016)
  • Author(s): Kobayashi K, Kato K.
  • Journal Title: Parasitol. Int. Volume: 65 Issue: 5 Pages: 549-551
  • DOI: 10.1016/j.parint.2016.09.002
  • Peer Reviewed / Open Access
• [Journal Article] Dynamics and plasticity of the epithelial to mesenchymal transition induced by miR-200 family inhibition (2016)
  • Author(s): Haraguchi, T, Kondo, M, Uchikawa, R, Kobayashi, K, Hiramatsu, H, Kobayashi, K , Chit, UW, Shimizu, T, Iba, H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep21117
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] MiR-199a inhibits secondary envelopment of HSV-1 by suppressing a Golgi-localized GAP for Cdc42 (2015)
  • Author(s): 小林郷介
  • Organizer: 第63回日本ウイルス学会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Antiviral host miRNA, miR-199a, regulates Cdc42 activity on the Golgi to inhibit HSV-1 secondary envelopment (2015)
  • Author(s): 小林郷介
  • Organizer: 第14回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県淡路市）
  • Year and Date: 2015-09-08
• [Presentation] HSV-1の2次エンベロープ形成を阻害する宿主miRNAの標的因子の研究 (2015)
  • Author(s): 小林郷介
  • Organizer: 第2回感染コンピテンシー若手研究会
  • Place of Presentation: マホロバマインズ三浦（神奈川県三浦市）
  • Year and Date: 2015-07-17
• [Presentation] 宿主miRNAによるウイルス増殖阻害に関する研究 (2015)
  • Author(s): 小林郷介
  • Organizer: 第12回ウイルス学キャンプ
  • Place of Presentation: ニューウェルシティ湯河原（静岡県熱海市）
  • Year and Date: 2015-05-23