| Project/Area Number |
15K19108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science (2016)
The University of Tokyo (2015) |
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Principal Investigator |
KOBAYASHI Kyousuke 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (80644989)
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| Research Collaborator |
IBA Hideo 千葉大学, 真菌医学研究センター, 教授
SAGARA Hiroshi 東京大学, 医科学研究所, 助教
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ヘルペスウイルス / miRNA / ゴルジ体 / エンベロープ形成 / ウイルス学 / Rho GTPase / ウイルス |
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| Outline of Final Research Achievements |
miRNAs regulate gene expression post transcriptionally. MiR-199a-3p, a kind of miRNA, reportedly impairs replication of various viruses. These evidences suggested that miR-199a-3p targets host factors important for replication of various virus. We here aimed to identify such host factors targeted by miR-199a using Herpes simplex virus-1 (HSV-1) as a model system. We identified human ARHGAP21 as a target of both miR-199a-5p and miR-199a-3p. Our results suggested that this molecule regulate the function of the Golgi apparatus and secondary envelopment of HSV-1 at the trans cisternae of the Golgi apparatus.
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