Function of Japanese encephalitis genome circularization on viral replication

• Project/Area Number: 15K19115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Dokkyo Medical University
• Principal Investigator: Ishikawa Tomohiro 獨協医科大学, 医学部, 助教 (40609327)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 日本脳炎ウイルス / ゲノム環状化 / 相補性 / 複製 / RNA / RNA複製 / ウイルスタンパク翻訳

Outline of Final Research Achievements

Functional analyzes of four pairs of complement sequences found in Japanese encephalitis virus (JEV) genome terminus were conducted to elucidate the effect of the genome circularization on virus replication. As results, essential sequences and/or complementarity for genome circularization as well as virus replication were determined. These regions possessed identical functions both in mammalian cells and mosquito cells. The essential sequences and/or complementarity for genome circularization were all important for virus replication. On the other hand, the essential sequences and/or complementarity for virus replication were not always essential for genome circularization. Since functions of these regions of JEV genome have been largely unknown, findings obtained in the present study can contribute to the novel anti JEV drug development which has not been available yet.

Research Products

• [Journal Article] Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum (2016)
  • Author(s): Keisuke Tabata, Masaru Arimoto, Masashi Arakawa, Atsuki Nara, Kazunobu Saito, Hiroko Omori, Arisa Arai, Tomohiro Ishikawa, Eiji Konishi, Ryosuke Suzuki, Yoshiharu Matsuura, Eiji Morita
  • Journal Title: Cell Reports Volume: 16 Issue: 9 Pages: 2339-2347
  • DOI: 10.1016/j.celrep.2016.07.068
  • Peer Reviewed / Open Access
• [Journal Article] Potential chemotherapeutic targets for Japanese encephalitis: current status of antiviral drug development and future challenges (2015)
  • Author(s): Ishikawa T and Konishi E
  • Journal Title: Expert Opinion on Therapeutic Targets Volume: 19 Issue: 10 Pages: 1379-1395
  • DOI: 10.1517/14728222.2015.1065817
  • Peer Reviewed
• [Presentation] 最近分離された遺伝子型V型に対する現行日本脳炎ワクチンの免疫原性 (2017)
  • Author(s): 本荘せいら、増田道明、石川知弘
  • Organizer: 第52回日本脳炎ウイルス生態学研究会
• [Presentation] Immunogenicity of the current Japanese encephalitis virus vaccine against recent genotype V strains (2017)
  • Author(s): Ishikawa T, Honjo S, Masuda M
  • Organizer: 第64回日本ウイルス学会学術集会
• [Presentation] 日本への侵入が懸念されるV型日本脳炎ウイルスに対するワクチン開発に向けて (2017)
  • Author(s): 本荘せいら、増田道明、石川知弘
  • Organizer: 第54回日本臨床生理学会総会
• [Presentation] Complementary sequences in the terminal regions of Japanese encephalitis virus genome play an essential role in viral RNA replication (2016)
  • Author(s): Tomohiro Ishikawa, Sarah Honjo, Michiaki Masuda
  • Organizer: 第64回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2016-10-23
• [Presentation] 日本脳炎ウイルスゲノムの環状化配列がウイルス複製に及ぼす影響 (2016)
  • Author(s): 石川知弘、本荘せいら、増田道明
  • Organizer: 第51回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテルリステル猪苗代（福島県）
  • Year and Date: 2016-05-13
• [Presentation] Development of novel virus-like particles useful for flavivirus studies (2015)
  • Author(s): Tomohiro Ishikawa, Takayuki Matsui, Michiaki Masuda
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2015-11-22
• [Presentation] 外来遺伝子を発現する日本脳炎ウイルスレプリコンの構築とその応用 (2015)
  • Author(s): 石川知弘、松井隆之、増田道明
  • Organizer: 第50回日本脳炎ウイルス生態学研究会
  • Place of Presentation: ホテル京都エミナース（京都府・京都市）
  • Year and Date: 2015-05-15