Regulation of HBs antigen expression in HBV genotype A infection

• Project/Area Number: 15K19120
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: YAMAMOTO Naoki 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主任研究員 (10547780)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ウイルス抗原発現制御

Outline of Final Research Achievements

Hepatitis B virus (HBV) antigens are involved in the suppression of the immune response to the virus. HBs antigens in cells infected with genotype A (Gt-A) virus were more abundantly expressed than those in cells infected with genotype C (Gt-C) virus. In the present study, transcription and translation efficiency were examined to analyze the regulatory mechanisms of HBs antigen expression. The results revealed that the transcription activities of the S1 and S2 promoters derived from Gt-A and Gt-C are comparable. In addition, no significant difference was observed in translation efficiency. These results suggest that HBs antigen expression is regulated after translation.

Research Products

• [Journal Article] Sato Y, Matsui H, Yamamoto N, Sato R, Munakata T, Kohara M, Harashima H. Highly specific delivery of siRNA to hepatocytes circumvents endothelial cell-mediated lipid nanoparticle-associated toxicity leading to the safe and efficacious decrease in the hepatitis B virus (2017)
  • Author(s): Sato Y, Matsui H, Yamamoto N, Sato R, Munakata T, Kohara M, Harashima H
  • Journal Title: J Control Release Volume: 266 Pages: 216-225
  • DOI: 10.1016/j.jconrel.2017.09.044
  • Peer Reviewed
• [Journal Article] Novel pH-sensitive multifunctional envelope-type nanodevice for siRNA-based treatments for chronic HBV infection (2016)
  • Author(s): Yamamoto, N. Sato, Y. Munakata, T. Kakuni, M. Tateno, C. Sanada, T. Hirata, Y. Murakami, S. Tanaka, Y. Chayama, K. Hatakeyama, H. Hyodo, M. Harashima, H. Kohara, M.
  • Journal Title: Journal of hepatology Volume: 64 Issue: 3 Pages: 547-455
  • DOI: 10.1016/j.jhep.2015.10.014
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] HBV持続感染に最適な培養系の構築 (2017)
  • Author(s): 山本直樹、棟方翼、小原道法
  • Organizer: 第65回日本ウイルス学会学術集会
• [Presentation] 新規肝臓特異的pH応答性DDS(MEND/HBV-siRNAmix)を用いたHBV持続感染の制御 (2016)
  • Author(s): 山本直樹
  • Organizer: 第26回抗ウイルス療法学会
  • Place of Presentation: 名古屋東急ホテル、名古屋市立大学大学院医学研究科(愛知県名古屋市)
  • Year and Date: 2016-05-13
• [Presentation] siRNA-based therapies to chronic HBV infection using a novel pH-sensitive multifunctional envelope-type nanodevice (2015)
  • Author(s): Naoki Yamamoto, Yusuke Sato, Tsubasa Munakata, Takahiro Sanada, Masakazu Kakuni, Chise Tateno, Shuko Murakami, Yasuhito Tanaka, Kazuaki Chayama, Hideyoshi Harashima, Michinori Kohara
  • Organizer: 2015 International Meeting on Molecular Biology of Hepatitis B Viruses
  • Place of Presentation: Bad Nauheim (Germany)
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Remarks] 公益財団法人 東京都医学総合研究所ホームページ
  • URL: http://www.igakuken.or.jp/
• [Remarks] 公益財団法人 東京都医学総合研究所
  • URL: http://www.igakuken.or.jp/