Role of CD28 co-stimulation in homeostasis of peripherally-derived regulatory T cells

• Project/Area Number: 15K19137
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Tokyo University of Science
• Principal Investigator: Wakamatsu Ei 東京理科大学, 研究推進機構生命医科学研究所, 助教 (40632617)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 制御性T細胞 / CD28 / 末梢由来制御性T細胞 / 分化 / 増殖 / シグナル

Outline of Final Research Achievements

Regulatory T cells (Tregs) are classified into thymus-derived Tregs and peripherally-derived Tregs (pTreg). It has not been fully understood how CD28 influences on homeostasis of pTreg. Thus, we attempted to investigate the role of CD28 in homeostasis of pTreg. We found that pTreg was significantly reduced in secondary lymphoid organs (SLOs) of CD28 knockout mice. And we clarified that the reduction of pTreg in SLOs of CD28 KO mice was due to the impairment of proliferation. Our study indicate that CD28 regulates proliferation of pTreg, leading to the maintenance of homeostasis of pTreg.

Research Products

• [Presentation] The responsiveness to TCR stimulation of Treg subsets influences the dependency of their proliferation on CD28 (2017)
  • Author(s): 若松英
  • Organizer: 第7回 International Kyoto T cell Conference
  • Place of Presentation: 芝蘭会館（京都府・京都市）
  • Year and Date: 2017-03-13
  • Int'l Joint Research
• [Presentation] 細胞増殖へのCD28シグナルの効果は制御性T細胞T細胞サブセット間で異なる (2016)
  • Author(s): 若松英
  • Organizer: 第26回 Kyoto T cell Conference
  • Place of Presentation: 芝蘭会館（京都府・京都市）
  • Year and Date: 2016-05-20
• [Presentation] CD28 makes different impacts on thymus-and peripherally-derived regulatory T cell for their maintenance. (2015)
  • Author(s): Wakamatsu E, Ohmori H, Ogawa S, Abe R
  • Organizer: The 44th Annual Meeting of The Japanese Society for Immunology
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2015-11-18
• [Presentation] CD28 co-stimulation is dispensable for the development of peripherally-derived regulatory T cells. (2015)
  • Author(s): Ohmori H, Wakamatsu E, Kawano A, Tabata Y, Ogawa S, Abe R
  • Organizer: The 44th Annual Meeting of The Japanese Society for Immunology
  • Place of Presentation: 札幌、札幌コンベンションセンター
  • Year and Date: 2015-11-18
• [Presentation] Role of CD28 co-stimulation in the homeostasis of peripherally derived regulatory T cells. (2015)
  • Author(s): Wakamatsu E, Ohmori H, Akieda Y, Ogawa S, Abe R
  • Organizer: 25th Kyoto T Cell Conference
  • Place of Presentation: 京都、京都大学芝蘭会館
  • Year and Date: 2015-05-15