Gene editing of patient-derived iPS cells by Zinc finger nuclease to analyze development of juvenile myelomonocytic leukemia
| Project/Area Number |
15K19177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUDA Kazuyuki 信州大学, 医学部附属病院, 主任臨床検査技師 (00647084)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | ZFN / JMML / PTPN11 / 若年性骨髄単球性白血病 / iPS細胞 / ジンクフィンガーヌクレアーゼ / ヌクレオフェクション / 遺伝子改変 |
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| Outline of Final Research Achievements |
We performed gene correction using Zinc Finger Nuclease (ZFN) specific for PTPN11 gene in juvenile myelomonocytic leukemia (JMML)-derived iPS cells with mutated PTPN11. PCR and direct sequencing confirmed that the mutated nucleotide was converted into wild-type one precisely. We will introduce mutation into PTPN11 wild-type iPS cells by the same protocol. The two types of gene-edited iPS cells (mutant-to-wild and wild-to-mutant) are useful for analysis of the cell kinetics to reveal the mechanism of development of JMML.
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Report
(3 results)
Research Products
(2 results)
