Functional analysis and clinical application of tumor-derived exosomes in adult T-cell Leukemia

• Project/Area Number: 15K19183
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Nagasaki University
• Principal Investigator: SASAKI Daisuke 長崎大学, 病院(医学系), 技術職員 (90624784)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: exosome / HTLV-1 / ATL / エクソソーム / miRNA

Outline of Final Research Achievements

In this study, we aimed to investigate the role of intercellular crosstalk mediated by miRNA contained in exosome secreted from ATL cells on ATL onset and progression. Exosomes were derived from plasma of HTLV-1 carrier and untreated ATL patient, comprehensive expression analysis of miRNA using microarray was performed. The miRNA profiles in ATL cells and miRNA profiles in exosomes were significantly different and it became clear that the miRNA profiles in exosomes derived from healthy human, HTLV-1 carrier and ATL patient plasma also had slight differences. This slight differences were thought that there is a possibility that it affects various cells.