The investigation of effects by antibiotics on spread of ESBL-producing bacteria worldwide.

• Project/Area Number: 15K19255
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Osaka Institute of Public Health
• Principal Investigator: Yamaguchi Takahiro 地方独立行政法人 大阪健康安全基盤研究所, 微生物部, 研究員 (80553635)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: プラスミド / 耐性菌 / コリスチン耐性遺伝子 / 薬剤耐性菌 / ESBL産生菌 / 抗菌性物質 / プラスミド性コリスチン耐性遺伝子 / コリスチン耐性 / ベトナム / 抗菌薬

Outline of Final Research Achievements

We investigated the effect of antibiotics on horizontal plasmid transduction using ESBL/AmpC producing E. coli were isolated from meats and fish and shellfish in Vietnam. As a result of the study, it was shown that the number of colonies of the transconjugants can be increased by adding a certain concentration at the time of conjugation in tetracycline and ciprofloxacin. Next, studies on the colistin resistance gene of ESBL / AmpC producing E. coli 261 strains revealed that 62 strains have plasmid harboring mcr-1 or mcr-3 and that plasmids are propagated by horizontal transmission. In addition, genetic analysis was performed on the plasmid having the mcr gene and its details were clarified.

Research Products

• [Journal Article] The Presence of Colistin resistance gene mcr-1 and -3 in ESBL producing Escherichia coli isolated from food in Ho Chi Minh City, Vietnam (2018)
  • Author(s): Yamaguchi T., Kawahara R., Harada K., Teruya S., Nakayama T., Motooka D., Nakamura S., Nguyen D P., Kumeda Y.,Dang V C.,Hirata K.,Yamamoto Y.
  • Journal Title: FEMS Microbiology Letters Volume: 印刷中 Issue: 11 Pages: 1-8
  • DOI: 10.1093/femsle/fny100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ベトナムの豚肉から分離したmcr-3を有するコリスチン耐性大腸菌の解析 (2018)
  • Author(s): ・山口貴弘、河原隆二、照屋志帆乃、原田和生、元岡大祐、中村昇太、中山達哉、Nguyen Do Phuc、Dang Van Chinh、久米田裕子、山本容正、平田收正
  • Organizer: 第91回 日本細菌学会総会
• [Presentation] ベトナム・ホーチミンの食品から分離したコリスチン耐性大腸菌の解析 (2017)
  • Author(s): 山口貴弘 河原隆二 原田和生 Nguyen Do Phuc Dang Van Chinh 久米田裕子 山本容正 平田收正
  • Organizer: 第９０回 日本細菌学会総会
  • Place of Presentation: 仙台市
  • Year and Date: 2017-03-19