Impact of anto-antibody in the development of abdominal aortic aneurysm

Research Project

Project/Area Number	15K19284
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General internal medicine(including psychosomatic medicine)
Research Institution	Osaka University
Principal Investigator	Nakagami Futoshi 大阪大学, 医学部附属病院, 助教 (60739176)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	腹部大動脈瘤 / 自己抗体 / 加齢 / Angiotensin II / 抗体 / 高齢
Outline of Final Research Achievements	Ang II infusion (1 ng/ml/min) for four weeks successfully induced aneurysm in only old mice (1-1.5 years old), but not in young mice (6-8 weeks) and the diameter of the abdominal aorta in old mice was significantly dilated compared to young mice. As an antibody injection model, the antibodies of old or young mice serum are isolated by protein G column and injected to young mice followed by Ang II infusion for four weeks. The old mice antibodies group elicit the development of abdominal aneurysm, but young group did not. The enzymatically fragmented old mice antibodies injection could not elicit aneurysm. And the old mice antibodies injection to C3 knock out mice could not induce aneurysm. These suggest the complement system works as the key role to elicit aneurysm in this model. Taken together, aged mice antibody may have the ability to induce an abdominal aneurysm to activate the complement system.