Endoscopic ultrasound-guided through-the-needle-biopsy for the diagnosis of pancreatic cystic neoplasms

• Project/Area Number: 15K19301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: Kawakubo Kazumichi 北海道大学, 医学研究科, 助教 (80633578)
• Research Collaborator: MITSUHASHI Tomoko 北海道大学病院, 病理診断部/病理部, 准教授 ; MIZUKAMI Yusuke 旭川医科大学, 内科学講座・消化器/血液腫瘍制御内科学分野, 准教授 ; ONO Yusuke 札幌東徳洲会病院, 付属臨床研究センター・臨床生体情報解析部 ; KUWATANI Masaki 北海道大学病院, 光学医療診療部, 助教
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵嚢胞性腫瘍 / EUS-FNA / 膵のう胞性腫瘍

Outline of Final Research Achievements

Endoscopic ultrasonography-guided through-the-needle cyst wall biopsy (EUS-TNCB) was performed in two patients. A 71-year-old female had 32mm cystic lesion in the head of the pancreas. EUS-TNCB revealed fragmented epithelial cells with small nucleus with interstitial fibrotic tissue. A 48-year-old male had 20mm cystic lesion in the body of the pancreas. EUS-TNCB revealed no epithelial cells but interstitial fibrotic tissue. Gene mutation analysis by next-generation sequencer could not detect any mutation in both samples.

Research Products

• [Journal Article] Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis. (2016)
  • Author(s): Kawakubo K, Ohnishi S, Fujita H, Kuwatani M, Onishi R, Masamune A, Takeda H, Sakamoto N.
  • Journal Title: Pancreas Volume: 45 Issue: 5 Pages: 707-713
  • DOI: 10.1097/mpa.0000000000000541
  • NAID: 120006220027
  • Peer Reviewed
• [Journal Article] Randomized trial comparing a side-port needle and standard needle for EUS-guided histology of pancreatic lesions (2016)
  • Author(s): Ishiwatari H, Hayashi T, Kawakami H, Isayama H, Hisai H, Itoi T, Ono M, Kawakubo K, Yamamoto N, Tanaka M, Itokawa F, Oshiro H, Sonoda T, Hasegawa T
  • Journal Title: Gastrointest Endosc Volume: 84 Issue: 4 Pages: 670-678
  • DOI: 10.1016/j.gie.2016.03.1329
  • Peer Reviewed
• [Journal Article] Lower incidence of complications in endoscopic nasobiliary drainage for hilar cholangiocarcinoma. (2016)
  • Author(s): Kawakubo K, Kawakami H, Kuwatani M, Haba S, Kudo T, Taya YA, Kawahata S, Kubota Y, Kubo K, Eto K, Ehira N, Yamato H, Onodera M, Sakamoto N.
  • Journal Title: World J Gastrointest Endosc. Volume: 10 Issue: 9 Pages: 385-490
  • DOI: 10.4253/wjge.v8.i9.385
  • Peer Reviewed / Open Access
• [Journal Article] Endoscopic ultrasound-guided fine-needle aspiration skill acquisition of gastrointestinal submucosal tumor by trainee endoscopists: A pilot study (2016)
  • Author(s): Niimi K, Goto O, Kawakubo K, Nakai Y, Minatsuki C, Asada-Hirayama I, Mochizuki S, Ono S, Kodashima S, Yamamichi N, Isayama H, Fujishiro M, Koike K.
  • Journal Title: Endosc Ultrasound. Volume: 5 Issue: 3 Pages: 157
  • DOI: 10.4103/2303-9027.183970
  • Peer Reviewed / Open Access
• [Journal Article] Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study. (2016)
  • Author(s): Kawakubo K, Ohnishi S, Hatanaka Y, Hatanaka KC, Hosono H, Kubota Y, Kamiya M, Kuwatani M, Kawakami H, Urano Y, Sakamoto N.
  • Journal Title: Mol Imaging Biol. Volume: 18 Issue: 3 Pages: 463-471
  • DOI: 10.1007/s11307-015-0898-5
  • NAID: 120006219913
  • Peer Reviewed
• [Journal Article] No Benefit of Oral Diclofenac on Post-Endoscopic Retrograde Cholangiopancreatography Pancreatitis (2016)
  • Author(s): Ishiwatari H, Urata T, Yasuda I, Matsusaki S, Hisai H, Kawakami H, Ono M, Iwashita T, Doi S, Kawakubo K, Hayashi T, Sonoda T, Sakamoto N, Kato J.
  • Journal Title: Dig Dis Sci. Volume: 61 Issue: 11 Pages: 3292
  • DOI: 10.1007/s10620-016-4251-x
  • Peer Reviewed
• [Presentation] Anti-inflammatory effects of human amnion-derived mesenchymal stem cell transplantation on rats with chronic pancreatitis (2016)
  • Author(s): Kawakubo K, Ohnishi S, Sakamoto N
  • Organizer: JDDW2016
  • Place of Presentation: 神戸コンベンションセンター(兵庫県神戸市)
  • Year and Date: 2016-11-03
• [Presentation] LIQUID BIOPSY FOR EARLY DETECTION OF PANCREATIC CANCER (2016)
  • Author(s): Mizukami Y, Ono Y, Karasaki H, Ogata M, Sugitani A, Koizumi K, Asahara S, Kawakubo K, Takahashi K, Maguchi H, Nagashima K
  • Organizer: APA2016
  • Place of Presentation: ボストン(米国)
  • Year and Date: 2016-10-26
  • Int'l Joint Research
• [Presentation] Which do you like?Side-by-side or stent-in-stent placement. (2016)
  • Author(s): Kawakubo K
  • Organizer: T-CAP 2016
  • Place of Presentation: 伊藤国際学術センター(東京都文京区)
  • Year and Date: 2016-06-17
  • Int'l Joint Research / Invited
• [Presentation] Risk of Bleeding After Endoscopic Ultrasnography-Guided Fine Needle Aspiration for Patients With Antithrombotic Agents; a Prospective Multicenter Study (2016)
  • Author(s): Kawakubo K, Yane K, Eto K, Ishiwatari H, Ehira N, Haba S, Matsumoto R, Shinada K, Yamato H, Kudo T, Onodera M, Okuda T, Taya Y, Kawahata S, Kubo K, Kubota Y, Kuwatani M, Kawakami H, Katanuma A, Ono M, Hayashi T, Uebayashi M, Sakamto N
  • Organizer: DDW2016
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2016-05-21
  • Int'l Joint Research