| Project/Area Number |
15K19307
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | プロバイオティクス / ポリリン酸 / エンドサイトーシス / タイトジャンクション |
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| Outline of Final Research Achievements |
The present study investigated the mechanism which Lactobacillus brevis-derived poly P exhibited the beneficial function. An immunostaining indicated that poly P was captured to plasma membrane via integrin β1 in caco2/bbe cells. The uptake of poly P was reduced by the inhibition of integrin β1 as well as caveolin-1, which a major component of lipid rafts. The function of poly P, including the induction of Hsp27 and enhancement of the intestinal barrier function, was suppressed by the inhibition of caveolin-1, illustrating that the function of poly P was mediated by the endocytic pathway. High-throughput sequencing revealed poly P induced tumor necrosis factor alpha-induced protein 3 (TNFAIP3), which contributes to the cytoprotection including upregulation of intestinal barrier function.
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