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Investigation of a novel host-microbial interaction focusing on the recognition of bacterial derived molecules by intestinal epithelial integrin.

Research Project

Project/Area Number 15K19307
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionAsahikawa Medical College

Principal Investigator

TANAKA Kazuyuki  旭川医科大学, 大学病院, 医員 (30624176)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsプロバイオティクス / ポリリン酸 / エンドサイトーシス / タイトジャンクション
Outline of Final Research Achievements

The present study investigated the mechanism which Lactobacillus brevis-derived poly P exhibited the beneficial function. An immunostaining indicated that poly P was captured to plasma membrane via integrin β1 in caco2/bbe cells. The uptake of poly P was reduced by the inhibition of integrin β1 as well as caveolin-1, which a major component of lipid rafts. The function of poly P, including the induction of Hsp27 and enhancement of the intestinal barrier function, was suppressed by the inhibition of caveolin-1, illustrating that the function of poly P was mediated by the endocytic pathway. High-throughput sequencing revealed poly P induced tumor necrosis factor alpha-induced protein 3 (TNFAIP3), which contributes to the cytoprotection including upregulation of intestinal barrier function.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (2 results)

All 2016 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Probiotic-derived polyphosphate improves the intestinal barrier function through the caveolin-dependent endocytic pathway.2015

    • Author(s)
      Tanaka K, Fujiya M, Konishi H, Ueno N, Sasajima J, Moriichi K, Ikuta K, Tanabe H, Kohgo Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 467 Issue: 3 Pages: 541-548

    • DOI

      10.1016/j.bbrc.2015.09.159

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 腸上皮エンドサイトーシスによる麦芽乳酸菌由来ポリリン酸の取り込みを介した腸管バリア機能増強作用の解析2016

    • Author(s)
      田中一之
    • Organizer
      第53回日本消化器免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-07-15
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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