New stomach cancer model mouse using Cre expression H. pylori

Research Project

Project/Area Number	15K19315
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Gastroenterology
Research Institution	The Institute for Adult Diseases Asahi Life Foundation
Principal Investigator	Nobumi Suzuki 公益財団法人朝日生命成人病研究所, その他部局等, 教授(移行) (30723746)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	ピロリ菌 / 胃癌
Outline of Final Research Achievements	It is a well-known that H. pylori infection causes stomach cancer. On the other hand, H. pylori that can not infect C57BL / 6 mice, which is the source of genetically modified mice. This was a obstacle in mouse experiments. Recently it has been reported that PMSS 1 strain can infect. We confirmed that the PMSS 1 strain can infect B6 mice. Then we made the gastritis mice model by useing PMSS1 strain and we reported a paper that IL - 1 signal and SOX 9 are involved in intestinal metaplasia. Subsequently, PMSS 1 strain did not express Cre, but we make CagA of PMSS1 to be KO and we confirmed activation of stress MAPK was dependent on CagA.