| Project/Area Number |
15K19325
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
IKEZAWA Kenji
SATO Katsuhiko
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 膵癌 / 前癌病変 / 新規モデルマウス / ５－ALA / 膵発癌マウス |
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| Outline of Final Research Achievements |
KrasLSL-G12D/+ Elastase1Cre Trp53flox/flox mice (KrasG12D-p53 deleted mice) showed pancreatic intraepithelial lesions (PanINs) and pancreatic ductal adenocarcinoma (PDAC) in 3 months. X-gal staining positive areas of the pancreatic tissue in Elastase1Cre crossing with ROSA26 mice was about 1-2% in whole pancreas. Positive areas of X-gal staining in the pancreas of KrasG12D-p53 deleted mice completely matched pancreatic tumors, whereas negative area of X-gal staining was morphologically normal acini (non-tumor lesions). We examined fluorescent positive areas of pancreatic frozen sections using fluorescence microscopy in KrasG12D-p53 deleted mice with intravenous injection of 5-ALA, and compared these fluorescent positive areas to histological findings of serial frozen sections with H&E staining. Positive fluorescent areas completely matched not only PDAC but also PanINs which are considered pre-cancerous lesions.
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