Identification and functional analysis of novel demethylation genes in the serrated pathway.
| Project/Area Number |
15K19333
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Research Collaborator |
MUGURUMA Naoki 徳島大学, 病院, 准教授
TAKAYAMA Tetsuji 徳島大学, 大学大学院医歯薬学研究部, 教授
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 大腸鋸歯状病変 / 脱メチル化 / SSA/P / S100P / メチル化 |
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| Outline of Final Research Achievements |
Methylation array analysis was performed using SSA/P samples and cancer in SSA/P samples, and we identified four genes(S100P, PKP3, MUC2, S100A2) as demethylation genes common to SSA/P. Each mRNA level was found to be increased by TaqMan PCR. Only Protein expression of S100P was markedly enhanced by immunostaining. We found that 30% of the promoter exist in exon 1 and intron 1 was demethylated. Knockdown experiments using siRNA of S100P gene were performed on the colon cancer cell line (HT29, WiDr) or SSA/P organoid culture cells, and cell proliferation and invasion ability were significantly suppressed in each cell.
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Report
(3 results)
Research Products
(2 results)
