| Project/Area Number |
15K19340
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Yokohama City University |
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Principal Investigator |
OGAWA Yuji 横浜市立大学, 医学部, 助教 (20644959)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | lipotoxicity / NAFLD / NASH / palmitate / saturated fatty acid / 飽和脂肪酸 / NAFLD/NASH |
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| Outline of Final Research Achievements |
In this study, after palmitate-injection, basal diet (BD)-fed mice did not altered the serum ALT, but high fat diet (HFD)-fed mice exhibited increased the serum ALT. Very low dose lipopolysaccharide (LPS)-injection increased the plasma endotoxin on BD-fed mice as same as HFD-fed mice. Both palmitate and very low dose LPS increased ALT on BD-fed mice. On BD-fed mice, palmitate induced inflammatory cells infiltration due to chemokines without ALT elevation and induced mild liver fibrosis via toll like receptor 4 (TLR4) pathway.
Collectively, palmitate alone induced inflammatory cells infiltration and mild liver fibrosis without ALT elevation. Furthermore, palmitate exacerbated NAFLD pathogenesis by cooperative interaction with gut-derived endotoxin. Our results indicate that serum palmitate and plasma gut-derived endotoxin reduction are important for NAFLD treatment.
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