Elucidation of the role of microRNA-27b for the development of NASH

• Project/Area Number: 15K19341
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Yokohama City University
• Principal Investigator: IMAJO Kento 横浜市立大学, 附属病院, 助教 (30600192)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 非アルコール性脂肪肝炎 / microRNA-27b / NASH / 腸内細菌 / エンドトキシン

Outline of Final Research Achievements

In this study, we have clarified that hepatic inflammation induced by hyper responsivity to endotoxin in fatty liver causes lipid excretion and metabolic disturbance by elevating microRNA - 27b (mir-27b), resulting in lipid metabolism disorder and accumulation of free fatty acid (FFA) which causes the development of NASH.

Research Products

• [Journal Article] Resveratrol ameliorates fibrosis and inflammation in a mouse model of nonalcoholic steatohepatitis. (2016)
  • Author(s): Kessoku T, Imajo K, Honda Y, et al.
  • Journal Title: Sci Rep Volume: 25 Issue: 1 Pages: 22251-22251
  • DOI: 10.1038/srep22251
  • NAID: 120005749754
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Selective SGLT2 Inhibitor Ipragliflozin Has a Therapeutic Effect on Nonalcoholic Steatohepatitis in Mice. (2016)
  • Author(s): Honda Y, Imajo K, Kato T, et al.
  • Journal Title: PLoS One Volume: 11 Issue: 1 Pages: e0146337-e0146337
  • DOI: 10.1371/journal.pone.0146337
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Magnetic Resonance Imaging More Accurately Classifies Steatosis and Fibrosis in Nonalcoholic Fatty Liver Disease Than Transient Elastography. (2016)
  • Author(s): Imajo K, Kessoku T, Honda Y, Tomeno W, Ogawa Y, Mawatari H, Fujita K, Yoneda M, Taguri M, Hyogo H, Sumida Y, Ono M, Eguchi Y, Inoue T,Yamanaka T, Wada K, Saito S, Nakajima A
  • Journal Title: Gastroenterology Volume: 150 Issue: 3 Pages: 626-37
  • DOI: 10.1053/j.gastro.2015.11.048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Are platelets count useful for detecting the grade of steatosis? (2015)
  • Author(s): Imajo K, Yoneda M, Nakajima A.
  • Journal Title: Hepat Mon. Volume: 23 Issue: 5 Pages: 626-637
  • DOI: 10.5812/hepatmon.15(5)2015.28957
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 腸管透過性を介した非アルコール性脂肪肝炎の病態進展における腸内細菌叢とエンドトキシンの関連性の検討 (2016)
  • Author(s): 結束貴臣
  • Organizer: 日本消化器免疫学会総会
  • Place of Presentation: ホテルニューオータニ大阪（大阪）
  • Year and Date: 2016-07-14
• [Presentation] 非アルコール性脂肪肝疾患患者の腸内細菌叢に関する検討 (2016)
  • Author(s): 結束貴臣
  • Organizer: 日本肝臓学会総会
  • Place of Presentation: 東京ベイ幕張ホール（千葉）
  • Year and Date: 2016-05-19
• [Presentation] Characteristics of fecal microbiota in nonalcoholic fatty liver disease (NAFLD) patients. (2016)
  • Author(s): Takaomi Kessoku, Kento Imajo, Atsushi Nakajima.
  • Organizer: The 51st European Association of the Study of Liver Disease Annual Meeting, parallel session, Barcelona, 2016, 04.
  • Place of Presentation: Barcelona（Spain）
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Magnetic Resonance Imaging More Accurately Classifies Steatosis and Liver Fibrosis in Patients with Nonalcoholic Fatty Liver Disease than Transient Elastography. (2016)
  • Author(s): Kento Imajo, Yasushi Honda, Takaomi Kessoku, Atsushi Nakajima.
  • Organizer: The 51st European Association of the Study of Liver Disease Annual Meeting, parallel session, Barcelona, 2016, 04.
  • Place of Presentation: Barcelona（Ｓｐａｉｎ）
  • Year and Date: 2016-04-15
  • Int'l Joint Research
• [Presentation] Overexpression of microRNA-27b develops the steatosis and insulin resistance (2015)
  • Author(s): Takaomi Kessoku, Kento Imajo, Atsushi Nakajima
  • Organizer: Japan Digestive Disease Week 2015
  • Place of Presentation: Vienna（Ａｕｓｔｒｉａ）
  • Year and Date: 2015-09-27
  • Int'l Joint Research