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High- throughput screening for personalized medicine targeting gastrointestinal cancer stem cell.

Research Project

Project/Area Number 15K19349
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Gastroenterology
Research InstitutionKeio University

Principal Investigator

TAKANO AI  慶應義塾大学, 医学部(信濃町), 研究員 (50647584)

Research Collaborator SATO Toshiro  慶應義塾大学, 医学部, 特任准教授 (70365245)
MATANO Mami  慶應義塾大学, 医学部, 研究員 (20439889)
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords消化器がん / HTS / 大腸癌 / がん幹細胞 / オルガノイド / PKI / スクリーニング / 幹細胞
Outline of Final Research Achievements

We have established gastrointestinal cancer organoids from the patient. As a result, we succeeded in isolating 4 kinds of tumor organoids including colorectal, gastric , liver, and pancreatic cancer from patient. It was possible to continuously and long-term culture of each of the human gastrointestinal cancer organoids by optimizing the culture condition based on the orgnoid culture protocol. We also have been successful knocked-in reporter to LGR5 locus of intestinal organoids using genome editing. Each gastrointestinal cancer organoids derived from patients became culture in 384-well plate. Furthermore, we have established tumor organoids; based High-throughput drug screen system. Image analysis was performed with 360 drugs on tumor organoids using High Contents Analyzer. Our results suggest that our established HTS for tumor organids will be apply to genetic mutation based drug discovery and personalized medicine.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Book (1 results)

  • [Journal Article] Visualization and Targeting of LGR5+ Human Colon Cancer Stem Cells2017

    • Author(s)
      Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T
    • Journal Title

      Nature

      Volume: 印刷中 Issue: 7653 Pages: 13-14

    • DOI

      10.1038/nature22081

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A colorectal tumor organoid library demonstrates progressive loss of niche factor requirements.2016

    • Author(s)
      Fujii M, Shimokawa M, Date S, Takano A, Matano M, Ohta Y, Nanki K, Kawasaki K, Nakazato Y, Uraoka T, Watanabe T, Kanai T, Sato T.
    • Journal Title

      Cell Stem Cell

      Volume: 18 Issue: 6 Pages: 827-838

    • DOI

      10.1016/j.stem.2016.04.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements2016

    • Author(s)
      Masayuki Fujii,Mariko Shimokawa,Shoichi Date,Ai Takano, Mam iMatano,Kosaku Nanki,Yuki Ohta,Kohta Toshimitsu,YoshihiroNakazato,Kenta Kawasaki,Toshio Uraoka,Toshiaki Watanabe,Takanori Kanai,Toshiro Sato
    • Journal Title

      Cell Stem Cell

      Volume: in press

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Modelling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids.2015

    • Author(s)
      Matano M,Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T.
    • Journal Title

      Nature Medicine

      Volume: 21(3) Issue: 3 Pages: 256-262

    • DOI

      10.1038/nm.3802

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Book] 細胞工学別冊 2015年上半期バイオ論文総集編2015

    • Author(s)
      高野愛, 佐藤俊朗
    • Total Pages
      2
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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