Development of novel treatment for ameliorating atherosclerosis by conditioned medium from mesenchymal stem cells
| Project/Area Number |
15K19403
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Takafuji Yoshimasa 国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (90734864)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 間葉系幹細胞 / 培養上清 / 動脈硬化 / エクソソーム / 血管内皮細胞 / マクロファージ / 抗炎症 / 炎症 / 間葉系幹細胞培養上清 / プラーク |
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| Outline of Final Research Achievements |
Intravenous administration of conditioned medium from cultured MSCs (MSC-CM) decreased the atherosclerotic lesions in model mouse. MSC-CM decreased the expression of cell adhesion molecules in endothelial cells (ECs), and decreased the expression of M1 markers and increased the expression of M2 markers in bone marrow derived macrophages. The anti-inflammatory action against ECs were induced by both proteins and exosomes in MSC-CM via inhibition of MAPK and NFkB pathway, and the M1/M2 polarization of macrophages were induced by proteins in MSC-CM via activation of STAT3 pathway and inhibition of MAPK pathway. The results of this study suggest that humoral factors from MSCs are promising for suppression of atherosclerosis by anti-inflammatory actions.
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Report
(3 results)
Research Products
(7 results)
