The role of chemokine CCL1 in experimental pulmonary emphysema induced by smoking exposure.

• Project/Area Number: 15K19411
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Yamagata University
• Principal Investigator: KIMURA Tomomi 山形大学, 医学部, 医員 (50536935)
• Research Collaborator: Shibata Yoko ; Sato Masamichi ; Sato Kento ; Nemoto Takako
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 慢性閉塞性肺疾患 / 肺気腫 / CCL1 / 喫煙 / 動物モデル / COPD

Outline of Final Research Achievements

Wild type mice 7 to 9 weeks old and SPC - CCL 1 Tg mice were exposed by cigarette smoking. After 1 week and 2 weeks, bronchoalveolar lavage (BAL) was performed, and total cell number and cell fraction were examined. In BAL fluid two weeks later, the total number of cells was significantly increased in the SPC-CCL1 Tg mice than in the wild type mice. The majority of the cellular fraction was alveolar macrophage. It was revealed that macrophages were more strongly induced by smoking exposure in SPC-CCL1 Tg mice. These results suggested that CCL1 might play a major role in the differentiation and induction of alveolar macrophages, which plays a central role in inflammatory response in the lung due to smoking exposure.

Research Products

• [Journal Article] Prevalence of diabetes mellitus in individuals with airflow obstruction in a Japanese general population: The Yamagata-Takahata Study. (2018)
  • Author(s): Machida H, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Sato K, Nakano H, Nishiwaki M, Kobayashi M, Yang S, Minegishi Y, Furuyama K, Yamamoto T, Watanabe T, Konta T, Ueno Y, Kato T, Kayama T, Kubota I
  • Journal Title: Respir Investig. Volume: 56 Issue: 1 Pages: 34-39
  • DOI: 10.1016/j.resinv.2017.11.003
  • Peer Reviewed
• [Journal Article] MafB enhances efferocytosis in RAW264.7 macrophages by regulating Axl expression. (2018)
  • Author(s): Sato M, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Nemoto T, Sato K, Nakano H, Abe S, Nishiwaki M, Kobayashi M, Yang S, Minegishi Y, Furuyama K, Kubota I
  • Journal Title: Immunobiology. Volume: 223 Issue: 1 Pages: 94-100
  • DOI: 10.1016/j.imbio.2017.10.007
  • Peer Reviewed
• [Journal Article] MafB silencing in macrophages does not influence the initiation and growth of lung cancer induced by urethane. (2017)
  • Author(s): Nemoto T, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Sato M, Sato K, Nakano H, Abe S, Nishiwaki M, Kobayashi M, Yang S, Minegishi Y, Furuyama K, Machida H, Kubota I
  • Journal Title: EXCLI Journal Volume: 16 Pages: 914-920
  • Peer Reviewed / Open Access
• [Journal Article] Decreased left ventricular stroke volume is associated with low-grade exercise tolerance in patients with chronic obstructive pulmonary disease. (2017)
  • Author(s): Inoue S, Shibata Y, Kishi H, Nitobe J, Iwayama T, Yashiro Y, Nemoto T, Sato K, Sato M, Kimura T, Igarashi A, Tokairin Y, Kubota I.
  • Journal Title: BMJ Open Respir Res. Volume: 4 Issue: 1 Pages: e000158-e000158
  • DOI: 10.1136/bmjresp-2016-000158
  • Peer Reviewed / Open Access
• [Journal Article] MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression. (2017)
  • Author(s): Nemoto T, Shibata Y, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Sato M, Sato K, Nakano H, Abe S, Nishiwaki M, Kubota I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 482 Issue: 2 Pages: 375-381
  • DOI: 10.1016/j.bbrc.2016.11.070
  • Peer Reviewed
• [Presentation] 肺炎患者における日常生活動作レベル低下の予測因子の検討 (2017)
  • Author(s): 峯岸幸博，井上純人，佐藤建人，佐藤正道，根本貴子，木村友美，山内啓子，五十嵐朗，東海林佳兼，柴田陽光
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] COPD増悪時の予後とシスタチンCとの関連 (2017)
  • Author(s): 佐藤建人，古山広大，峯岸幸博，梁秀鼎，中野寛之，佐藤正道，根本貴子，木村友美，山内啓子，五十嵐朗，東海林佳兼，井上純人，柴田陽光，久保田功
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] 血清E-selectin濃度と炎症性肺疾患の予後との関連について (2017)
  • Author(s): 中野寛之，井上純人，五十嵐朗，東海林佳兼，山内啓子，木村友美，根本貴子，佐藤正道，佐藤建人，梁秀鼎，古山広大，峯岸幸博，八木橋崇仁，久保田功，柴田陽光
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] 非小細胞肺癌における病期と血清ヘプシジンとの関連 (2017)
  • Author(s): 佐藤建人，古山広大，峯岸幸博，梁秀鼎，中野寛之，佐藤正道，根本貴子，木村友美，山内啓子，五十嵐朗，東海林佳兼，井上純人，柴田陽光，久保田功
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] 下気道感染症患者における血清LYVEI濃度の解析 (2017)
  • Author(s): 佐藤正道，柴田陽光，井上純人，五十嵐朗，東海林佳兼，山内啓子，木村友美，根本貴子，佐藤建人，中野寛之，梁秀鼎，古山広大，峯岸幸博，久保田功
  • Organizer: 第57回日本呼吸器学会学術講演会
• [Presentation] Serum level of lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) during acute lower respiratory infection (2017)
  • Author(s): Nakano H, Shibata Y , Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Nishiwaki M, Nemoto T, Sato M, Sato K, Yan S, Furuyama K, Minegishi Y, Kubota I
  • Organizer: American thoracic Society International Conference2017
  • Int'l Joint Research
• [Presentation] Association of cystayin C-based estimated glomerular filtration rate (eGFR) with prognosis in patients with COPD exacerbation (2017)
  • Author(s): Machida H, Huruyama K, Minegishi Y, Yang S, Sato K, Nakano H, Kobayashi M, Sato M, Nemoto T, Nishiwaki M, Kimura T, Yamauchi K, Igarashi A, Tokairin Y, Inoue S, Kubota I, Shibata Y
  • Organizer: American thoracic Society International Conference2017
  • Int'l Joint Research
• [Presentation] Association of smaller erector spinae muscle size on admission with unrecovered activities of daily living after pneumonia treatment (2017)
  • Author(s): Yukihiro Minegishi, Sumito Inoue, Kento Sato, Kohdai Furuyama, Sujeong Yang, Hiroshi Nakano, Masamichi Sato, Takako Nemoto, Tomomi Kimura, Keiko Yamauchi, Akira Igarashi, Yoshikane Tokairin, Yoko Shibata
  • Organizer: American thoracic Society International Conference2017
  • Int'l Joint Research