The role of proton-sensing ovarian cancer G protein-coupled receptor 1 on dendritic cells and T cells in a murine asthma model

• Project/Area Number: 15K19412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Gunma University
• Principal Investigator: Saito Haruka 群馬大学, 医学部附属病院, 医員 (80447268)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: アレルギー / 気管支喘息

Outline of Final Research Achievements

Ovarian cancer G protein-coupled receptor 1 (OGR1) stimulation by extracellular protons causes the activation of G proteins and subsequent cellular functions. Expression and the role of OGR1 at the cell level were unknown. We confirmed OGR1 mRNA expression assessed by real-time TaqMan PCR in peribronchial lymph node, CD４+Tcell and CD8+Tcell from splenocyte of WT mice. OGR1 developed in the peribronchial lymph node which I gathered from house dust induced bronchial asthma model. Furthermore, OGR1 developed in isolated CD4+T cell, CD8+T cell by magnetic beads from a splenocyte.

Research Products

• [Journal Article] Elemental and immunohistochemical analysis of the lungs and hilar lymph node in a patient with asbestos exposure, a pilot study. (2016)
  • Author(s): Koga Y, Satoh T, Kaira K, Koka M, Hisada T, Hirato J, Altan B, Yatomi M, Ono A, Kamide Y, Shimizu Y, Aoki-Saito H, Tsurumaki H, Shimizu K, Mogi A, Ishizuka T, Yamada M, Dobashi K.
  • Journal Title: Environ Health Prev Med. Volume: 6 Issue: 6 Pages: 492-500
  • DOI: 10.1007/s12199-016-0576-5
  • NAID: 50010159091
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] CREB regulates TNF-α-induced GM-CSF secretion via p38 MAPK in human lung fibroblasts (2016)
  • Author(s): Koga Y, Hisada T, Ishizuka T, Utsugi M, Ono A, Yatomi M, Kamide Y, Aoki-Saito H, Tsurumaki H, Dobashi K, Yamada M.
  • Journal Title: Allergology International Volume: 65 Issue: 4 Pages: 406-413
  • DOI: 10.1016/j.alit.2016.03.006
  • NAID: 130005431338
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protective role of proton-sensing TDAG8 in lipopolysaccharide-induced acute lung injury. (2015)
  • Author(s): H. Tsurumaki, C. Mogi, H. Aoki-Saito, M. Tobo, Y. Kamide, M. Yatomi, K. Sato, K. Dobashi, T. Ishizuka, T. Hisada, M. Yamada, F. Okajima
  • Journal Title: Int. J. Mol. Sci. Volume: 16 Issue: 12 Pages: 28931-28942
  • DOI: 10.3390/ijms161226145
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 17(R)-resolvin D1 ameliorates bleomycin-induced pulmonary fibrosis in mice. (2015)
  • Author(s): Yatomi M, Hisada T, Ishizuka T, Koga Y, Ono A, Kamide Y, Seki K, Aoki-Saito H, Tsurumaki H, Sunaga N, Kaira K, Dobashi K, Yamada M, Okajima F.
  • Journal Title: PHYSIOLOGICAL REPORTS Volume: 3 Issue: 12 Pages: 12628-12628
  • DOI: 10.14814/phy2.12628
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Acidic environment augments FcεRI-mediated production of IL-6 and IL-13 in mast cells. (2015)
  • Author(s): Y. Kamide, T. Ishizuka, M. Tobo, H. Tsurumaki, H. Aoki, C. Mogi, T. Nakakura, M. Yatomi, A. Ono, Y. Koga, K. Sato, T. Hisada, K. Dobashi, M. Yamada, F. Okajima
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 464 Issue: 3 Pages: 949-955
  • DOI: 10.1016/j.bbrc.2015.07.077
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Absence of proton-sensing OGR1 protects against house dust mite-induced allergic airway inflammation (2016)
  • Author(s): Haruka Saito, Takeshi Hisada, Chihiro Mogi, Tsurumaki Hiroaki, Masakiyo Yatomi, Yosuke Kamide, Akihiro Ono, Yasuhiko Koga, Kunio Dobashi, Tamotsu Ishizuka, Fumikazu Okajima, Masanobu Yamada.
  • Organizer: ERS（欧州呼吸器学会）INTERNATIONAL CONGRESS 2016
  • Place of Presentation: London, united kingdom
  • Year and Date: 2016-09-03
• [Presentation] ハウスダストマイト点鼻気管支喘息モデルにおけるプロトン感知性受容体OGR1の役割 (2016)
  • Author(s): 齋藤（青木）悠、茂木千尋、鶴巻寛朗、矢冨正清、上出庸介、古賀康彦、小野昭浩、土橋邦生、石塚全、岡島史和、久田剛志
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2016-06-18
• [Presentation] ハウスダストマイト点鼻気管支喘息モデルにおけるプロトン感知性受容体OGR1の役割 (2016)
  • Author(s): ハウスダストマイト点鼻気管支喘息モデルにおけるプロトン感知性受容体OGR1の役割
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京国際フォーラム（東京）
  • Year and Date: 2016-06-17
• [Presentation] T細胞におけるプロトン感知性受容体OGR1の役割 (2016)
  • Author(s): 齋藤（青木）悠、茂木千尋、鶴巻寛朗、久田剛志、小野昭浩、古賀康彦、関香織、矢冨正清、上出庸介、土橋邦生、石塚全、山田正信、岡島史和
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 京都国際会議場（京都）
  • Year and Date: 2016-04-08
• [Presentation] TDAG8はLPS誘導性急性肺損傷において抑制的に働く (2016)
  • Author(s): 鶴巻寛朗、齋藤（青木） 悠、茂木千尋、久田剛志、石塚全、上出庸介、矢冨正清、古賀康彦、小野昭浩、佐藤幸市、土橋邦生、山田正信、岡島史和
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 京都国際会議場（京都）
  • Year and Date: 2016-04-08
• [Presentation] T細胞におけるプロトン感知性受容体OGR1の役割 (2016)
  • Author(s): 齋藤（青木）悠、茂木千尋、鶴巻寛朗、久田剛志、小野昭浩、古賀康彦、関香織、矢冨正清、上出庸介、土橋邦生、石塚全、山田正信、岡島史和
  • Organizer: 第56回日本呼吸器学会学術講演会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2016-04-08