Development of personalized therapy targeting circulating tumor cells with cancer stem cell properties

• Project/Area Number: 15K19418
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: Hase Tetsunari 名古屋大学, 医学部附属病院, 病院助教 (30621635)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 循環腫瘍細胞 / 肺癌幹細胞

Outline of Final Research Achievements

Circulation tumor cells (CTCs) were isolated from patients with lung cancer. Epidermal growth factor receptor (EGFR) mutations and MET amplification were detected successfully in single CTC using whole genome amplification technology. Also, gene expression analysis and PDL1 expression in single CTC were done successfully.
On the basis of these results, we prepared clinical trials for evaluation of molecular mechanisms of resistance to targeted therapies.