Functional analysis for apical transporters by gene silencing in isolated and cultured renal proximal tubules.
| Project/Area Number |
15K19441
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 水・電解質代謝学 / ナトリウム－プロトン交換輸送体 / ＮＨＥ / 腎臓内科学 / 水電解質代謝学 / ナトリウム輸送 / エンドサイトーシス / NHE活性 / V-ATPase活性 / 近位尿細管 |
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| Outline of Final Research Achievements |
We aimed to explore the detailed mechanisms of sodium dependent acid base transport in the apical side of renal proximal tubules (PTs). Cell pH measurement was performed in freshly isolated PTs with several inhibitors including EIPA and DIDS, or in cultured PTs which was gene silenced with siRNA. Consequently, we revealed the existence of DIDS-sensitive components other than Na/H exchanger 3 in the apical side of PTs, which was a possible key in species differences of sodium dependent acid base transport among mice, rats and humans.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] A pure chloride channel mutant of CLC-5 causes Dent’s disease via insufficient V-ATPase activation2016
Author(s)
Nobuhiko Satoh, Hideomi Yamada, Osamu Yamazaki, Masashi Suzuki, Motonobu Nakamura, Atsushi Suzuki, Akira Ashida, Daisuke Yamamoto, Yoshitsugu Kaku, Takashi Sekine, George Seki, Shoko Horita
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Journal Title
Pfluegers Aｒch
Volume: 印刷中
Issue: 7
Pages: 1183-96
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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