New mechanism of cardio-renal association: Evaluation of the effect of megalin inhibition to the atherosclerosis through the renal protection

• Project/Area Number: 15K19447
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: Kaseda Ryohei 新潟大学, 医歯学総合病院, 助教 (20737697)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: アポEノックアウトマウス / メガリンノックアウトマウス / 心腎連関 / メガリン・アポEダブルノックアウトマウス / インドキシル硫酸 / アポＥノックアウトマウス

Outline of Final Research Achievements

Megalin・ApoE double KO mice have been successfully established. 21-week-old Megalin・ApoE double KO mice have a tendency to have decreased atherosclerotic lesion of the aortas compared with ApoE KO mice. In our studies to evaluate the mechanisms of atherosclerosis in kidney injury, we have investigated the impact of nutrition intake.After 10-week-old Apo E KO mice underwent uninephrectomy, these mice were pair-fed with a usual casein (animal protein)-based diet or rice protein-based diet for 7 weeks. Compared with the animal protein-based diet, the plant protein-based diet significantly reduced atherosclerosis accelerated by renal mass reduction.