Elucidation of the immune target molecules in chronic inflammatory demyelinating polyneuropathy by proteomic analysis.

• Project/Area Number: 15K19475
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Chiba University
• Principal Investigator: BEPPU MINAKO 千葉大学, 大学院医学研究院, 助教 (70623669)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 慢性炎症性脱髄性多発神経炎 / プロテオミクス解析 / 自己抗体

Outline of Final Research Achievements

Chronic inflammatory demyelinating polyneuropathy (CIDP) is an autoimmune disorder of peripheral nervous system. Current hypothesis is that humoral immune factors contribute to the autoimmune mechanisms. The aim of this study was to identify the immunological target antigens in subgroups of CIDP by proteomic-based approach. Target molecules of autoantibodies in the patient’s sera were investigated among extracted proteins from pig cauda equine by agarose 2-DE and immunoblotting. The candidate molecules were validated by immunoblotting, immunohistochemistry and immunocytochemistry.We found that some patients with CIDP had anti-vinculin autoantibodies. Vinculin exists ubiquitously and plays a key role in the cell adhesion.Our results suggested that vinculin could be a possible immunological target of autoantibodies in a few patients with typical CIDP.

Research Products

• [Journal Article] Autoantibodies against vinculin in patients with chronic inflammatory demyelinating polyneuropathy. (2015)
  • Author(s): Beppu M, Sawai S, Satoh M, Mori M, Kazami T, Misawa S, Shibuya K, Ishibashi M, Sogawa K, Kado S, Kodera Y, Nomura F, Kuwabara S.
  • Journal Title: J Neuroimmunol. Volume: 287 Pages: 9-15
  • DOI: 10.1016/j.jneuroim.2015.07.012
  • Peer Reviewed
• [Presentation] 平山病における血清サイトカインプロファイル解析 (2016)
  • Author(s): 別府美奈子 澤井摂 三澤園子 森雅裕 伊藤彰一 曽川一幸 西村基 松下一之 野村文夫 桑原聡
  • Organizer: 第５７回日本神経学会学術大会
  • Place of Presentation: 神戸国際展示場(兵庫県、神戸市）
  • Year and Date: 2016-05-18