| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We confirmed primary microglia under oxygen-glucose deprivation (OGD) conditions like mild ischemia polar to protective M2 microglia. We found that intraarterial administration of OGD microglia promoted functional recovery via angiogenesis in the border area within the ischemic core as well as axonal outgrowth in the ischemic penumbra by remodeling factors such as vascular endothelial growth factor (VEGF) and transforming growth factor at 28 days after ischemia. Furthermore, we could isolated primary microglia from adult rats under cerebral ischemia. We also demonstrated that OGD condition prompted more secretion of VEGF than hypoxia or glucose-deprivation stimulus only. In conclusion, intravascular administration of M2 microglia preconditioned by optimal OGD might be a novel therapeutic strategy against ischemic stroke.
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