The establishment of therapeutic strategies of microglial transplantation against chronic ischemic stroke
Project/Area Number |
15K19478
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Neurology
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Research Institution | Niigata University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
Shimohata Takayoshi 岐阜大学, 医学部, 教授 (60361911)
Takahashi Tetsuya 新潟大学, 医歯学総合病院, 助教 (20515663)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 脳梗塞 / ミクログリア / 細胞療法 / 血管新生 / 神経軸策伸展 / M2 / M2化 / 慢性期脳梗塞 |
Outline of Final Research Achievements |
We confirmed primary microglia under oxygen-glucose deprivation (OGD) conditions like mild ischemia polar to protective M2 microglia. We found that intraarterial administration of OGD microglia promoted functional recovery via angiogenesis in the border area within the ischemic core as well as axonal outgrowth in the ischemic penumbra by remodeling factors such as vascular endothelial growth factor (VEGF) and transforming growth factor at 28 days after ischemia. Furthermore, we could isolated primary microglia from adult rats under cerebral ischemia. We also demonstrated that OGD condition prompted more secretion of VEGF than hypoxia or glucose-deprivation stimulus only. In conclusion, intravascular administration of M2 microglia preconditioned by optimal OGD might be a novel therapeutic strategy against ischemic stroke.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Microglia preconditioned by oxygen-glucose deprivation promote functional recovery in ischemic rats2017
Author(s)
Masato Kanazawa, Minami Miura, Masafumi Toriyabe, Misaki Koyama, Masahiro Hatakeyama, Masanori Ishikawa, Takashi Nakajima, Osamu Onodera, Tetsuya Takahashi, Masatoyo Nishizawa, Takayoshi Shimohata
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 42582-42582
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] M2 microglia preconditioned by oxygen-glucose deprivation promotes functional recovery in ischemic rats2017
Author(s)
Masato Kanazawa, Minami Miura, Masafumi Toriyabe, Misaki Koyama, Masahiro Hatakeyama, Masanori Ishikawa, Takashi Nakajima, Osamu Onodera, Tetsuya Takahashi, Masatoyo Nishizawa, Takayoshi Shimohata
Organizer
International Stroke Conference 2017
Place of Presentation
Houston, TX, USA
Year and Date
2017-02-22
Related Report
Int'l Joint Research
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[Presentation] Preconditioned microglia by oxygen-glucose deprivation promote functional recovery in ischemic rats2017
Author(s)
Kanazawa M, Miura M, Toriyabe M, Koyama M, Hatakeyama M, Ishikawa M, Nakajima T, Onodera O, Takahashi T, Nishizawa M, Shimohata T
Organizer
8th Korea/Japan Stroke Conference
Related Report
Int'l Joint Research
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[Presentation] Preconditioned protective microglia by oxygen-glucose deprivation promote functional recovery in ischemic rats2017
Author(s)
Kanazawa M, Miura M, Toriyabe M, Koyama M, Hatakeyama M, Ishikawa M, Nakajima T, Onodera O, Takahashi T, Nishizawa M, Shimohata T
Organizer
World Congress of Neurology
Related Report
Int'l Joint Research
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[Presentation] Preconditioned M2 microglia by oxygen-glucose deprivation promote functional recovery in ischemic rats2017
Author(s)
Kanazawa M, Miura M, Toriyabe M, Koyama M, Hatakeyama M, Ishikawa M, Nakajima T, Onodera O, Takahashi T, Nishizawa M, Shimohata T
Organizer
Annual meeting of American Academy of Neurology
Related Report
Int'l Joint Research
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[Presentation] Microglia preconditioned by oxygen-glucose deprivation promote functional recovery in ischemic rats2017
Author(s)
Kanazawa M, Miura M, Toriyabe M, Koyama M, Hatakeyama M, Ishikawa M, Nakajima T, Onodera O, Takahashi T, Nishizawa M, Shimohata T
Organizer
XXVIIIth International Symposium on Cerebral Blood Flow, Metabolism
Related Report
Int'l Joint Research
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