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Genetic research on Parkinson's disease using medaka fish

Research Project

Project/Area Number 15K19486
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurology
Research InstitutionKyoto University

Principal Investigator

Uemura Norihito  京都大学, 医学研究科, 特定助教 (90749045)

Research Collaborator KINOSHITA Masato  京都大学, 大学院農学研究科, 助教
NAKANISHI Etsuro  京都大学, 大学院医学研究科, 大学院生
Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsパーキンソン病 / ゴーシェ病 / 自然免疫 / メダカ
Outline of Final Research Achievements

GBA mutations are the strong risk factor of Parkinson’s disease. We have developed GBA-/- medaka which display neuronal loss and alpha-synuclein accumulation accompanied with neuroinflammation. In this study, we analyzed the association of neuroinflammation with the pathophysiology in GBA-/- medaka. MyD88 and TRIF are the adaptor molecules of TLRs. We successfully established MyD88 and TRIF knock-out medaka by CRISPR/Cas9. MyD88-/- and TRIF-/- medaka did not show any apparent abnormal phenotypes. Therefore, we analyzed GBA-/-MyD88-/- and GBA-/-TRIF-/- medaka, but any improvement in the neuronal loss or life span was not observed in those double mutant medaka compared to GBA-/- medaka. These results suggest that signal pathways via MyD88 and TRIF do not associate with the pathophysiology in GBA-/- medaka.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Journal Article] メダカを用いたパーキンソン病研究2016

    • Author(s)
      上村紀仁
    • Journal Title

      実験医学(増刊)

      Volume: 34 Pages: 187-194

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Viable neuronopathic Gaucher disease model in medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein2015

    • Author(s)
      Norihito Uemura, Masato Koike, Satoshi Ansai, Masato Kinoshita, Tomoko Ishikawa-Fujiwara, Hideaki Matsui, Kiyoshi Naruse, Naoaki Sakamoto, Yasuo Uchiyama, Takeshi Todo, Shunichi Takeda, Hodaka Yamakado, Ryosuke Takahashi
    • Journal Title

      PLoS Genetics

      Volume: 11 Issue: 4 Pages: 1-14

    • DOI

      10.1371/journal.pgen.1005065

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] パーキンソン病の動物モデル2015

    • Author(s)
      上村紀仁
    • Journal Title

      Pharma medica

      Volume: 33 Pages: 25-29

    • Related Report
      2015 Research-status Report
  • [Journal Article] メダカのパーキンソン病モデル2015

    • Author(s)
      上村紀仁
    • Journal Title

      細胞工学

      Volume: 34 Pages: 1166-77

    • NAID

      40020669016

    • Related Report
      2015 Research-status Report
  • [Presentation] Viable neuronopathic Gaucher disease model in medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein2016

    • Author(s)
      Norihito Uemura, Ryosuke Takahashi
    • Organizer
      International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
    • Place of Presentation
      名古屋
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 神経型ゴーシェ病モデルメダカは軸索にアルファシヌクレイン蓄積を示す2016

    • Author(s)
      上村紀仁
    • Organizer
      日本放射線影響学会-第59回大会-
    • Place of Presentation
      広島
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経型ゴーシェ病メダカは軸索にアルファシヌクレイン蓄積を示す2015

    • Author(s)
      上村紀仁
    • Organizer
      BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Medaka fish model of Parkinson’s disease2015

    • Author(s)
      Norihito Uemura, Ryosuke Takahashi
    • Organizer
      10th GEO-PD
    • Place of Presentation
      東京
    • Year and Date
      2015-10-02
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Gaucher disease model in medaka displays axonal accumulation of alpha-synuclein2015

    • Author(s)
      Norihito Uemura
    • Organizer
      第58回日本神経化学会大会
    • Place of Presentation
      さいたま
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Gaucher disease model in medaka displays axonal accumulation of alpha-synuclein2015

    • Author(s)
      Norihito Uemura
    • Organizer
      第56回日本神経学会学術大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-05-20
    • Related Report
      2015 Research-status Report
  • [Book] エキスパートに学ぶパーキンソン病・パーキンソニズムQ&A2017

    • Author(s)
      髙橋良輔、上村紀仁他
    • Total Pages
      248
    • Publisher
      南山堂
    • Related Report
      2016 Annual Research Report
  • [Remarks] パーキンソン病の解明に役立つメダカの作製に成功 -メダカが神経変性疾患の研究に貢献できる可能性-

    • URL

      http://www.kyoto-u.ac.jp/ja/research/research_results/2015/150403_1.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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