| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We developed enzymatic histochemistry to detect granular osmiophilic material (GOM) deposits, which are specific pathologic findings of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Structures of mutated Notch3 proteins are similar to those of monoamine oxidase. We hypothesized that GOM deposits may have amine oxidase activity. We performed enzymatic histochemical staining to detect GOM deposits using unfixed frozen sections of skeletal muscles samples obtained from patients with CADASIL.
In this study, we revealed that GOM deposits were detected by enzymatic histochemical staining of amine oxidase activity. This method was useful to observe GOM deposits by light microscopy. It may be available to analyze enzymatic activity of GOM deposits and to select existent medicine that inhibits enzymatic activity of GOM deposits.
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