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Elucidation of PPY cell lineage for development of new beta cell origin

Research Project

Project/Area Number 15K19526
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Metabolomics
Research InstitutionJuntendo University

Principal Investigator

Hara Akemi  順天堂大学, 医学部, 研究員 (60570009)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
KeywordsPP細胞 / β細胞 / 糖尿病 / 細胞系譜 / 分化転換 / 膵ラ氏島 / PPY細胞
Outline of Final Research Achievements

In our previous study, we demonstrated the possibility that high fat diet and streptozotocin can induce beta to PPY cell fate conversion in adult pancreas. The aim of this study was to elucidate the mechanism of the fate conversion between PPY and beta cells for development of new beta cell origin. In this study, we used our original mouse monoclonal antibody with a stringent binding specificity for PP. We employed lineage traceable mice, which our original PPY- NLS- Cre knock- in mice were bred with Rosa26-YFP mice. By immunohistochemical study, at 4 weeks of age, majority of GFP positive cells expressed PPY in the periphery of islets. In 8 weeks of age, some GFP positive cells were not positive for PPY. In 16 weeks of age, more than half of GFP positive cells were not positive for PPY in core region of islets, which was mainly occupied by beta cells. Hence, we found that substantial number of beta cells in adult islets were originated from PPY cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2015

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Expression mechanism of tryptophan hydroxylase 1 in mouse islets during pregnancy.2015

    • Author(s)
      Iida H, Ogihara T, Min MK, Hara A, Kim YG, Fujimaki K, Tamaki M, Fujitani Y, Kim H, Watada H.
    • Journal Title

      J Mol Endocrinol

      Volume: 55 (1) Issue: 1 Pages: 41-53

    • DOI

      10.1530/jme-14-0299

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Human IAPP impairs the success of islet transplantation2015

    • Author(s)
      A. Hara, Y. Fujitani, T. Ogihara, T. Miyatsuka, H. Watada
    • Organizer
      51st EASD Annual Meeting
    • Place of Presentation
      Philadelphia
    • Year and Date
      2015-09-14
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト型IAPPは膵島移植の成功率を低下させる2015

    • Author(s)
      原 朱美、藤谷 与士夫、荻原 健、宮塚 健、綿田 裕孝
    • Organizer
      第58回日本糖尿病学会年次学術集会
    • Place of Presentation
      山口
    • Year and Date
      2015-05-21
    • Related Report
      2015 Research-status Report
  • [Patent(Industrial Property Rights)] 抗膵ポリペプチドモノクローナル抗体2015

    • Inventor(s)
      藤谷与士夫、綿田裕孝、原朱美、中尾啓子
    • Industrial Property Rights Holder
      藤谷与士夫、綿田裕孝、原朱美、中尾啓子
    • Industrial Property Rights Type
      特許
    • Filing Date
      2015-12-18
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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