Exploration of the mechanisms for supplying octanoic acid for ghrelin acylation in X/A-like cells.

• Project/Area Number: 15K19532
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Endocrinology
• Research Institution: Wakayama Medical University
• Principal Investigator: BANDO MIKA 和歌山県立医科大学, 医学部, 特別研究員 (70737874)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: グレリン / アシル化 / 長鎖脂肪酸 / 炎症 / IL-1

Outline of Final Research Achievements

The mechanisms for supplying octanoic acid for ghrelin acylation in X/A-like cells are incompletely understood. We found that long-chain fatty acids were incorporated at a higher rate in the ghrelin-producing cell line MGN3-1 than in MIN6 cells, in part due to higher expression level of long-chain fatty acyl-CoA synthetase family member 1 (Acsl1). Inhibition of ACSLs by triacsin C profoundly suppressed acylated ghrelin production. These results suggest that high incorporation of long-chain fatty acids into the ghrelin-producing cells plays a role in the supply of octanoic acid for ghrelin acylation.

Research Products

• [Journal Article] IL-1β directly suppress ghrelin mRNA expression in ghrelin-producing cells. (2017)
  • Author(s): Bando M, Iwakura H, Ueda Y, Ariyasu H, Inaba H, Furukawa Y, Furuta H, Nishi M, Akamizu T.
  • Journal Title: Mol Cell Endocrinol. Volume: 447 Pages: 45-51
  • DOI: 10.1016/j.mce.2017.02.032
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High incorporation of long-chain fatty acids contributes to the efficient production of acylated ghrelin in ghrelin-producing cells. (2016)
  • Author(s): Bando M, Iwakura H, Koyama H, Hosoda H, Shigematsu Y, Ariyasu H, Akamizu T, Kangawa K, Nakao K.
  • Journal Title: FEBS Lett. Volume: 590 Issue: 7 Pages: 992-1001
  • DOI: 10.1002/1873-3468.12132
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Establishment of Leptin-Responsive Cell Lines from Adult Mouse Hypothalamus. (2016)
  • Author(s): Iwakura H, Dote K, Bando M, Koyama H, Hosoda K, Kangawa K, Nakao K
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0148639-e0148639
  • DOI: 10.1371/journal.pone.0148639
  • NAID: 120005763397
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Comprehensive Profiling of GPCR Expression in Ghrelin-producing Cells. (2016)
  • Author(s): Koyama H, Iwakura H, Dote K, Bando M, Hosoda H, Ariyasu H, Kusakabe T, Son C, Hosoda K, Akamizu T, Kangawa K, Nakao K.
  • Journal Title: Endocrinology Volume: 157 Issue: 2 Pages: 692-704
  • DOI: 10.1210/en.2015-1784
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] グレリン発現調節への炎症性サイトカインの影響の検討 (2017)
  • Author(s): 坂東美佳、岩倉浩、上田陽子、赤水尚史
  • Organizer: 第90回日本内分泌学会学術総会
  • Place of Presentation: ロームシアター京都、京都市勧業館みやこめっせ（京都府京都市）
  • Year and Date: 2017-04-20
• [Presentation] 炎症性サイトカインのグレリン遺伝子発現への影響の検討 (2016)
  • Author(s): 坂東美佳、岩倉浩、上田陽子、赤水尚史
  • Organizer: 第37回日本肥満学会
  • Place of Presentation: 東京ファッションタウン（東京都江東区）
  • Year and Date: 2016-10-07
• [Presentation] LPS directly increased ghrelin and GOAT mRNA expressions via NF-kappaB signaling pathway in MGN3-1 cell (2015)
  • Author(s): Bando M, Iwakura H, Koyama H, Kangawa K, Nakao K.
  • Organizer: The Obesity Society’s 2015 Annual Scientific Meeting
  • Place of Presentation: Los Angeles(USA)
  • Year and Date: 2015-11-02
  • Int'l Joint Research
• [Presentation] Investigating the role of betatrophin in glucose and lipid metabolism using betatrophin knockout rats (2015)
  • Author(s): Kusakabe T, Izumi R, Noguchi M, Iwakura H, Koyama H, Bando M, Miyazawa T, Hosoda K, Kangawa K, Nakao K.
  • Organizer: Keystone Symposia Diabetes: New Insights into Molecular Mechanisms and Therapeutic Strategies (T2)
  • Place of Presentation: Kyoto(Japan)
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] グレリン産生におけるLPSの直接作用の検討 (2015)
  • Author(s): 坂東美佳、岩倉浩、小山博之、寒川賢治、中尾一和
  • Organizer: 第36回日本肥満学会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-02
• [Presentation] ベータトロフィンのノックアウトラット開発とその糖脂質代謝調節における意義の解明 (2015)
  • Author(s): 泉諒太、日下部徹、野口倫生、岩倉浩、小山博之、坂東美佳、宮澤崇、寒川賢治、中尾一和
  • Organizer: 第36回日本肥満学会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-02
• [Presentation] GPCR発現プロファイル解析に基づく摂食促進ホルモン、グレリン分泌調節機構の解明 (2015)
  • Author(s): 小山博之、岩倉浩、坂東美佳、細田洋司、細田公則、寒川賢治、中尾一和
  • Organizer: 第36回日本肥満学会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-02