| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Mammalian erythroblasts undergo enucleation through a process thought to be similar to cytokinesis. However, the mechanism of position determination of contraction ring in enucleation is still unknown.
We investigated the presence of various molecules involved in cell division on the proliferation of human colony-forming unis-erythroid and erythroblasts. Cdc42, mDia2 and mDia3 were involved determining the position of contraction ring formation during erythroblast enucleation. Furthermore, Cdc42 inhibitor blocked proliferation of CFU-Es in a dose-dependent manner and reduced the enucleation ratio of erythroblasts. These results suggest that Cdc42 plays an important role in both cytokinesis and cell polarization through the regulation of dynein and actin filament organization during terminal differentiation of human erythroblasts.
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