The functional difference among Pcgf family proteins in hematopoietic system
Project/Area Number |
15K19544
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Hematology
|
Research Institution | Chiba University |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ポリコーム / Pcgf1 / non-canonical PRC1 / エピジェネティクス / Non-canonical PRC1 / 造血幹細胞分化 / Pcgf3 / Pcgf4/Bmi1 / Pcgf5 |
Outline of Final Research Achievements |
We found that deletion of Pcgf1 in mice did not compromise self-renewal capacity of HSCs, but induced accumulation of granulocyte-macrophage progenitors and mature myeloid cells, eventually leading to the development of a lethal myeloproliferative neoplasm-like disease in mice. Detailed analysis of hematopoietic stem and progenitor cells (HSPCs) revealed skewing of HSC differentiation to myeloid lineages at the expense of B lymphocyte commitment in the absence of Pcgf1.Comprehensive analyses revealed that Cebpa, a master transcriptional factor for myeloid differentiation, was up-regulated following a reduction in H2AK119ub1 levels in Pcgf1-deficient HSPCs, resulting in the activation of C/EBPα targets in Pcgf1-deficient MPPs. These results indicate that Pcgf1 negatively regulates myeloid commitment of HSPCs to balance their multi-lineage differentiation
|
Report
(3 results)
Research Products
(7 results)
-
-
[Journal Article] Non-Lethal Ionizing Radiation Promotes Aging-Like Phenotypic Changes of Human Hematopoietic Stem and Progenitor Cells in Humanized Mice.2015
Author(s)
Wang C, Oshima M, Sashida G, Tomioka T, Hasegawa N, Mochizuki-Kashio M, Nakajima-Takagi Y, Kusunoki Y, Kyoizumi S, Imai K, Nakachi K, Iwama A.
-
Journal Title
PLoS One
Volume: 10
Issue: 7
Pages: e0132041-e0132041
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
[Presentation] Role of the polycomb gene Bcor in hematopoiesis2015
Author(s)
Tomoyuki Tanaka, Yaeko Nakajima-Takagi, Motohiko Oshima, Atsunori Saraya, Shiro Tara, Sha Si, Hirohito Sone, Haruhiko Koseki, Atsushi Iwama
Organizer
The 44th Annual Meeting of International Society for Experimental Hematology
Place of Presentation
国立京都国際会館(京都府, 京都市)
Year and Date
2015-09-17
Related Report
Int'l Joint Research