| Project/Area Number |
15K19549
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Research Collaborator |
SHIRAI Toshiaki
TSUKIJI Nagaharu
SHINMORI Hedeyuki
INOUE Katsue
OZAKI Yukio
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 血小板 / CLEC-2 / ロドサイチン |
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| Outline of Final Research Achievements |
Platelet activation receptor CLEC-2 binds to podoplanin expressed on the surface of several kinds of cancer cells, which facilitates cancer metastasis. Therefore, anti-CLEC-2 drugs are expected to have anti-platelet and anti-metastatic effects. We focused on snake venom rhodocytin as its drug discovery source. In order to verify the binding site of rhodocytin with CLEC-2 and the formation mechanism of the complex, recombinant wild-type and mutant rhodocytin were produced with mammalian cell line. As a result, we found mutant rhodocytin with anti-CLEC-2 effect. In the mouse model, this mutant rhodocytin strongly inhibited podoplanin-dependent cancer metastasis without inducing platelet aggregation.
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