Mechanical dissection of leukemic stem cell generation by using primate ES/iPS cell-derived hematopoietic stem/progenitor cells
| Project/Area Number |
15K19552
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kohara Hiroshi 東京大学, 医科学研究所, 特任講師 (40528733)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 白血病 / ES細胞 / iPS細胞 / がん遺伝子 / がん抑制遺伝子 / ヒトES細胞 |
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| Outline of Final Research Achievements |
Inducible gene expression cassette of oncogenes including KRAS G12V were successfully inserted into adeno-associated virus integration site 1 (AAVS1) locus of human ES cells and iPS cells by genome editing technique. CD34-positive hematopoietic cells were induced from these ES/iPS cells. After additional 7 day culture of these hematopoietic cells with KRAS G12V induction resulted in altered cell growth. Now CD34-positive hematopoietic cells were transplanted into irradiated immune-deficient mice in order to investigate in vivo competitive repopulation ability in comparison with normal hematopoietic cells derived from ES/iPS cells.
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Report
(3 results)
Research Products
(1 results)
