Mechanical dissection of leukemic stem cell generation by using primate ES/iPS cell-derived hematopoietic stem/progenitor cells

Research Project

Project/Area Number	15K19552
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Hematology
Research Institution	The University of Tokyo
Principal Investigator	Kohara Hiroshi 東京大学, 医科学研究所, 特任講師 (40528733)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	白血病 / ES細胞 / iPS細胞 / がん遺伝子 / がん抑制遺伝子 / ヒトES細胞
Outline of Final Research Achievements	Inducible gene expression cassette of oncogenes including KRAS G12V were successfully inserted into adeno-associated virus integration site 1 (AAVS1) locus of human ES cells and iPS cells by genome editing technique. CD34-positive hematopoietic cells were induced from these ES/iPS cells. After additional 7 day　culture of these hematopoietic cells with KRAS G12V induction resulted in altered cell growth. Now CD34-positive hematopoietic cells were transplanted into irradiated immune-deficient mice in order to investigate in vivo competitive repopulation ability in comparison with normal hematopoietic cells derived from ES/iPS cells.

Report

(3 results)