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The mechanism of drug resistance regulated by miR-155 in T-ALL

Research Project

Project/Area Number 15K19557
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionJichi Medical University

Principal Investigator

Koyama Daisuke  自治医科大学, 医学部, 客員研究員 (50741071)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsmiR-155 / bortezomib / T-ALL / 薬剤耐性 / マイクロRNA / プロテアソーム阻害剤 / hsa-miR-155
Outline of Final Research Achievements

We previously reported that bortezomib induces cell death and increases chemosensitivity via transcriptional repression of Notch1 in T-cell acute lymphoblastic leukemia. The down-regulation of Notch1 was caused by the degradation of Sp1. But the mechanism of Sp1 degradation is still unknown. We hypothesized that Sp1 is degraded by some microRNA. In order to find the microRNA targeting Sp1, we performed the microRNA array and identified that miR-155 was up-regulated by bortezomib. We confirmed it using CRISPR/Cas9 system. These results indicated that miR-155 could be the biomarker of the drug sensitivity for bortezomib.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (5 results)

All 2015 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Remarks (2 results)

  • [Journal Article] Phosphorylation-mediated EZH2 inactivation promotes drug resistance in multiple myeloma.2015

    • Author(s)
      Kikuchi J, Koyama D, Wada T, Izumi T, Hofgaad PO, Bogen B, Furukawa Y.
    • Journal Title

      J Clin Invest

      Volume: 125 Issue: 12 Pages: 4375-4390

    • DOI

      10.1172/jci80325

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Overexpression of the shortest isoform of histone demethylase LSD1 primes hematopoietic stem cells for malignant transformation.2015

    • Author(s)
      Wada T, Koyama D, Kikuchi J, Honda H, Furukawa Y.
    • Journal Title

      Blood

      Volume: 125 Issue: 24 Pages: 3731-3746

    • DOI

      10.1182/blood-2014-11-610907

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Soluble aKlotho as a Candidate for the Biomarker of Aging.2015

    • Author(s)
      Koyama, D., Sato, Y., Aizawa, M., Maki, T., Kurosawa, M., Kuro-o, M. and Furukawa, Y
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 467 Issue: 4 Pages: 1019-1025

    • DOI

      10.1016/j.bbrc.2015.10.018

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Remarks] 老化とくに骨粗鬆症の診断に有用な血中バイオマーカーの発見

    • URL

      https://www.jichi.ac.jp/news/research/2015/20151124.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 老化とくに骨粗鬆症の診断に有用な血中バイオマーカーの発見

    • URL

      http://www.jichi.ac.jp/openlab/newsletter/h46_spletter.pdf

    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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