Involvement of molecular chaperone in multistage carcinogenesis of HTLV-1-infected cells

• Project/Area Number: 15K19562
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Kawasaki Medical School
• Principal Investigator: Naito Tadasuke 川崎医科大学, 医学部, 助教 (50455937)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HTLV-1 / 分子シャペロン / Heat Shock Factor 1 / プロテオチューナー法 / 分子シャペロンタンパク質 / HSF1 / ATL / 遺伝子変異 / Tax

Outline of Final Research Achievements

To understand the effect of heat shock proteins and HSF1 (Heat shock factor 1) in multistage carcinogenesis of HTLV-1-infected cells, we performed the construction of an in vivo control system of HSF1 function using by ProteoTuner technology. As a result, we succeeded in the establishment of reversible expression system of HSF1-dominant active and HSF1-dominant negative mutants for the analysis of chaperone network function during HTLV-1 infection.

Research Products

• [Journal Article] Influenza A virus hemagglutinin and neuraminidase act as novel motile machinery (2017)
  • Author(s): Sakai, T. Nishimura, S. I. Naito,T. Saito, M.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45043-45043
  • DOI: 10.1038/srep45043
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Generation of a genetically stable high-fidelity influenza vaccine strain. (2017)
  • Author(s): Naito T, Mori K, Ushirogawa H, Takizawa N, Nobusawa E, Odagiri T, Tashiro M, Ohniwa RL, Nagata K, Saito M.
  • Journal Title: Journal of Virology Volume: 91 Issue: 6 Pages: 614-614
  • DOI: 10.1128/jvi.01073-16
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pre-mRNA Processing Factor Prp18 Is a Stimulatory Factor of Influenza Virus RNA Synthesis and Possesses Nucleoprotein Chaperone Activity. (2017)
  • Author(s): Minakuchi M, Sugiyama K, Kato Y, Naito T, Okuwaki M, Kawaguchi A, Nagata K.
  • Journal Title: Journal of Virology Volume: 91 Issue: 3
  • DOI: 10.1128/jvi.01398-16
  • Peer Reviewed / Open Access
• [Journal Article] Re-emergence of H3N2 strains carrying potential neutralizing mutations at the N-linked glycosylation site at the hemagglutinin head, post the 2009 H1N1 pandemic. (2016)
  • Author(s): Ushirogawa H, Naito T, Tokunaga H, Tanaka T, Nakano T, Terada K, Ohuchi M, Saito M.
  • Journal Title: BMC Infectious Diseases Volume: 16 Issue: 1 Pages: 380-380
  • DOI: 10.1186/s12879-016-1738-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Development of a high-yield reassortant influenza vaccine virus derived from the A/Anhui/1/2013 (H7N9) strain. (2016)
  • Author(s): Nakamura K, Shirakura M, Suzuki Y, Naito T, Fujisaki S, Tashiro M, Nobusawa E.
  • Journal Title: Vaccine Volume: 12 Issue: 3 Pages: 328-333
  • DOI: 10.1016/j.vaccine.2015.11.050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Absolute quantification of HTLV-1 basic leucine zipper factor (HBZ) protein and its plasma antibody in HTLV-1 infected individuals with different clinical status. (2016)
  • Author(s): Shiohama Y, Naito T, Matsuzaki T, Tanaka R, Tomoyose T, Takashima H, Fukushima T, Tanaka Y, Saito M.
  • Journal Title: Retrovirology Volume: 13 Issue: 1 Pages: 1-14
  • DOI: 10.1186/s12977-016-0263-z
  • NAID: 120006847302
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Molecular analysis of HTLV-1 subgroups associated with the development of HAM/TSP (2017)
  • Author(s): 内藤忠相、齊藤峰輝
  • Organizer: 18th International Conference on Human Retrovirology, HTLV and Related Viruses
  • Place of Presentation: ホテル グランドアーク半蔵門（東京都千代田区）
  • Year and Date: 2017-03-07
  • Int'l Joint Research
• [Presentation] ウイルスポリメラーゼの高忠実化誘導による変異の入りにくいインフルエンザワクチン母体株の開発 (2016)
  • Author(s): 内藤忠相
  • Organizer: 5th Negative Strand Virus-Japan Symposium
  • Place of Presentation: ホテルモントレ沖縄（沖縄県国頭郡）
  • Year and Date: 2016-01-25
• [Presentation] HAM発病関連ウイルス型がTaxおよびHBZの標的遺伝子発現に及ぼす影響の網羅的解析 (2015)
  • Author(s): 内藤忠相, 水戸部悠一, 安間恵子, 松岡雅雄, 齊藤峰輝
  • Organizer: 第2回日本HTLV-1学会学術集会
  • Place of Presentation: 東京大学医科学研究所（東京都港区）
  • Year and Date: 2015-08-22
• [Presentation] 高忠実化ポリメラーゼ誘導による低抗原変異性インフルエンザワクチン母体株の開発 (2015)
  • Author(s): 内藤忠相, 後川潤, 齊藤峰輝
  • Organizer: 第56回日本臨床ウイルス学会
  • Place of Presentation: 岡山大学（岡山県岡山市）
  • Year and Date: 2015-06-13
• [Remarks] 川崎医科大学微生物学教室のホームページ
  • URL: http://www.kawasaki-m.ac.jp/microbiology/