Suppression of B-cell antibody production with human LAG+ Treg and analysis of mechanism of TGF-beta 3
Project/Area Number |
15K19566
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Collagenous pathology/Allergology
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Research Institution | The University of Tokyo |
Principal Investigator |
Sumitomo Shuji 東京大学, 医学部附属病院, 助教 (20392996)
|
Research Collaborator |
TSUCHIDA Yumi
NAKACHI Shinichiro
KATO Rika
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Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 制御性T細胞 / B細胞 / 抑制性サイトカイン / TGF-β3 |
Outline of Final Research Achievements |
LAG3+ Treg identified by the research leaders specifically produces the inhibitory cytokine TGF-β 3 in mice and strongly suppresses antibody production of B cells. In this study, the mechanism of action of TGF-β3 on human primary B cells was elucidated. TGF-β3 inhibited antibody production of human primary B cells, induced cell death of human B cells, inhibited proliferation and inhibited differentiation into plasmablast cells. TGF-β3 suppressed gene expression such as IRF4, Blimp-1, and XBP1, which are important for differentiation into antibody-producing cells, and suppressed phosphorylation of Syk. In addition, in this study, human tonsillar and human peripheral blood LAG3+ Treg were analyzed and the relationship between LAG3+ Treg and rheumatoid arthritis, abatacept was clarified.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Identification of tonsillar CD4±CD25=LAG3± T cells as naturally occurring IL-10-producing regulatory T cells in human lymphoid tissue.2017
Author(s)
Sumitomo S, Nakachi S, Okamura T, Tsuchida Y, Kato R, Shoda H, Furukawa A, Kitahara N, Kondo K, Yamasoba T, Yamamoto K, Fujio K.
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Journal Title
Journal of autoimmunity.
Volume: 76
Pages: 75-84
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Abatacept facilitates differentiation of IL-10-producing-LAG3+ regulatory CD4+ T cells with antibody suppression effect.2017
Author(s)
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, Sakurai K, Hanata N, Nagafuchi Y, Tateishi S, Kanda H, Okamura T, Yamamoto K, Fujio K.
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Journal Title
Arthritis Research & Therapy
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses2015
Author(s)
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K.
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Journal Title
Nature Communications
Volume: 6
Issue: 1
Pages: 1-14
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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