Searching for a diagnostic marker and identification of important cell groups in non-IgE mediated gastrointestinal diseases

• Project/Area Number: 15K19580
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Waseda University
• Principal Investigator: Orihara Kanami 早稲田大学, 理工学術院, 講師(任期付) (60450623)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 好酸球性消化管疾患 / 非IgE依存性食物タンパク質誘発性胃腸炎 / リンパ球刺激試験 / 食物タンパク質誘発性胃腸炎 / 消化管アレルギー / 細胞群 / サイトカイン / 細胞増殖 / 血便 / 非IgE依存性アレルギー

Outline of Final Research Achievements

Non-IgE-mediated eosinophilic gastrointestinal diseases are the diseases that the number of patients continues to increase all over the world, pathology of which in particular in neonates to infants is difficult to distinguish from other diseases. Thus, sometimes it causes life-thretening serious complications.
Therefore, in this study, we aimed to test the practical utility of the antigen-specific lymphocyte stimulation test, which is conducted at the research level at the moment, if it can be clinically applied to the diagnosis of these diseases. As a result, it was confirmed that it can be used for diagnosis, whereas weakness remains in distinguishing these diesases from IgE-mediated (normal) food allergy. I also examined cytokines and proliferating cell sub-popiulations as applied analysis versions of antigen specific lymphocyte tests, and I was able to find differences between IgE-mediated food allergy and non-IgE-mediated eosinophilic gastrointestinal diseases.

Research Products

• [Journal Article] Eosinophilic esophagitis versus proton pump inhibitor-responsive esophageal eosinophilia: Transcriptome analysis (2017)
  • Author(s): Shoda T, Matsuda A, Nomura I, Okada N, Orihara K, Mikami H, Ishimura N, Ishihara S, Matsumoto K, Kinoshita Y
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: - Issue: 6 Pages: 2010-2013
  • DOI: 10.1016/j.jaci.2016.11.028
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ene expression patterns in distinct endoscopic findings for eosinophilic gastritis in children (2017)
  • Author(s): Sato M, Shoda T, Shimizu H, Orihara K, Futamura K, Matsuda A, Yamada Y, Yoshioka T, Shimizu T, Ohya Y, Nomura I, Matsumoto K, Arai K.
  • Journal Title: JACI In Practice Volume: in press
• [Journal Article] Sera of patients with infantile eosinophilic gastroenteritis showed a specific increase in both thymic stromal lymphopoietin and IL-33 levels (2016)
  • Author(s): Shoda T, Matsuda A, Arai K, Shimizu H, Morita H, Orihara K, Okada N, Narita M, Ohya Y, Saito H, Matsumoto K, Nomura I.
  • Journal Title: J Allergy Clin Immunol. Volume: in press Issue: 1 Pages: 299-303
  • DOI: 10.1016/j.jaci.2015.11.042
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Recent advances in understanding the roles of vascular endothelial cells in allergic inflammation (2016)
  • Author(s): Shoda T, Futamura K, Orihara K, Emi-Sugie M, Saito H, Matsumoto K, Matsuda A.
  • Journal Title: Allergology International Volume: 65 Issue: 1 Pages: 21-29
  • DOI: 10.1016/j.alit.2015.08.001
  • NAID: 130005290335
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access
• [Journal Article] Controlling the peripheral clock might be a new treatment strategy in allergy and immunology. (2016)
  • Author(s): Orihara K, Saito H.
  • Journal Title: J Allergy Clin Immunol. Volume: 137 Issue: 4 Pages: 1236-1237
  • DOI: 10.1016/j.jaci.2015.08.054
  • Peer Reviewed
• [Presentation] Stool EDN levels in different clusters of non-IgE-mediated gastrointestinal food allergy. (2017)
  • Author(s): Orihara K, Nomura I, Shoda T, Suzuki H, Morita H, Matsuda A, Saito H, Matsumoto K.
  • Organizer: The 73rd Annual Meeting of American Academy of Allergy, Asthma and Immunology
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 2017-03-03
  • Int'l Joint Research
• [Presentation] Perinatal characteristics of infants with non-IgE-mediated gastrointestinal food allergy. (2017)
  • Author(s): Nomura I, Suzuki H, Motomura K, Shoda T, Morita H, Orihara K, Ohya Y, Saito H, Matsumoto K.
  • Organizer: The 73rd Annual Meeting of American Academy of Allergy, Asthma and Immunology
  • Place of Presentation: Atlanta, GA, USA
  • Year and Date: 2017-03-03
  • Int'l Joint Research
• [Presentation] 新生児-乳児消化管アレルギー、クラスター3における血清中TSLP、IL33の上昇 (2016)
  • Author(s): 野村伊知郎、正田哲雄、松田明生、鈴木啓子、折原芳波、岡田直子、森田英明、成田雅美、大矢幸弘、斎藤博久、松本健治
  • Organizer: 第65回日本アレルギー学会
  • Place of Presentation: 東京、東京国際フォーラム
  • Year and Date: 2016-06-17
• [Presentation] Distinct plasma chemokine levels in non-IgE-mediated gastrointestinal food allergy, compared with IgE-mediated food allergy. (2016)
  • Author(s): Orihara K, Nomura I, Shoda T, Morita H, Suzuki H, Matsuda A, Saito H, Matsumoto K.
  • Organizer: The 31st Symposium of the Collegium Internationale Allergologicum
  • Place of Presentation: Charleston, SC, USA
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] Plasma cytokine/chemokine profiles in non-IgE-mediated gastrointestinal food allergy. (2016)
  • Author(s): Orihara K, Nomura I, Shoda T, Morita H, Suzuki H, Matsuda A, Saito H, Matsumoto K.
  • Organizer: 72nd Annual Meeting of American Academy of Allergy, Asthma and Immunology
  • Place of Presentation: Los Angeles, CA, USA
  • Year and Date: 2016-03-04
• [Presentation] 非IgE依存性消化管アレルギー患児におけるCRP上昇には抗原特異的なIL-6産生が関与する (2015)
  • Author(s): 折原芳波、野村伊知郎、正田哲雄、鈴木啓子、森田英明、松田明生、斎藤博久、松本健治
  • Organizer: 第52回日本小児アレルギー学会
  • Place of Presentation: 奈良
  • Year and Date: 2015-11-21
• [Presentation] IgE非依存性消化管アレルギーの病型ごとに抗原刺激リンパ球培養上清のサイトカインプロファイルは異なる (2015)
  • Author(s): 折原芳波、野村伊知郎、正田哲雄、森田英明、松田明生、斎藤博久、松本健治
  • Organizer: 第64回日本アレルギー学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-26
• [Book] 臨床免疫・アレルギー科 (2016)
  • Author(s): 折原芳波、松本健治
  • Total Pages: 5
  • Publisher: 科学評論社