Construction of human artificial chromosome towards practical use
| Project/Area Number |
15K19615
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Tottori University |
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Principal Investigator |
Uno Narumi 鳥取大学, 医学(系)研究科(研究院), 助教 (30733357)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ヒト人工染色体 / 微小核細胞融合法 / 染色体工学 / ゲノム編集 / 人工染色体 / CRISPR/Cas9 |
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| Outline of Final Research Achievements |
Human artificial chromosome vector (HAC) is promising for the development of gene- and cell-therapy for Duchenne muscular dystrophy, which is a genetic disorder with an X-linked recessive inheritance pattern . However, HAC should be developed and introduced to target cells in a condition without animal cells. In this study, we developed a chromosome modification technology for human chromosome in CHO or A9 cells of the model cells of somatic cells with CRISPR/Cas9, called genome editing tool. On the other hand, the chromosome modification technology in human cells was examined and chemical compounds was screened towards the development of microcell-mediated chromosome transferring with human cells as a donor of a chromosome. Although these studies did not showed an expected results, further consideration should be required towards the achievements of the development of these technologies.
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Report
(4 results)
Research Products
(6 results)
